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Effects of interleukin 3, interleukin 7, and B-cell growth factor on proliferation and drug resistance in vitro in childhood acute lymphoblastic leukemia

Authors :
A. E. J. Duyn
A. J. P. Veerman
C. H. Van Zantwijk
Gertjan J.L. Kaspers
Karel Hählen
Rob Pieters
G. J. Broekema
Pediatrics
Pediatric surgery
AII - Cancer immunology
CCA - Cancer biology and immunology
CCA - Cancer Treatment and quality of life
Source :
Annals of Hematology, 78, 163-171. Springer-Verlag, Annals of Hematology, 78(4), 163-171. Springer Verlag, Duyn, A E J, Kaspers, G J L, Pieters, R, Van Zantwijk, C H, Broekema, G J, Hählen, K & Veerman, A J P 1999, ' Effects of interleukin 3, interleukin 7, and B-cell growth factor on proliferation and drug resistance in vitro in childhood acute lymphoblastic leukemia ', Annals of Hematology, vol. 78, no. 4, pp. 163-171 . https://doi.org/10.1007/s002770050495
Publication Year :
1999

Abstract

Growth factors have been reported to enhance the cytotoxicity of anticancer agents. In our study we investigated the capacities of interleukin 3 (IL-3), interleukin 7 (IL-7), low-molecular-weight B-cell growth factor (Imw-BCGF), and IL-3 + 7 to induce proliferation and to modulate the drug resistance of childhood acute lymphoblastic leukemia (ALL) cells. Proliferation was assessed with the methyl-thiazole-tetrazolium (MTT) assay and other parameters. Cellular resistance to cytarabine, thioguanine, and prednisolone was measured using the MTT assay. In 19 samples containing > 90% leukemic cells the proliferative response and the modulation of drug resistance was markedly heterogeneous between patient samples and between growth factors. All growth factors were able to stimulate proliferation significantly after 5 days of culture. Imw-BCGF was the most potent growth factor in this respect. Cytotoxicity of cytarabine and thioguanine was significantly increased by IL-7, that of thioguanine by IL-3 as well. IL-7 enhanced the cytotoxicity of thioguanine significantly more than IL-3 and Imw-BCGF and that of cytarabine more than IL-3. Cytotoxicity of prednisolone was not significantly influenced by any growth factor. In individual cases, growth factors reduced the cytotoxicity of the drugs. IL-3 + 7 did not add activity to the most potent single growth factor in both proliferation and drug resistance measurements. This study shows that IL-3, IL-7, and Imw-BCGF generally induce and occasionally inhibit proliferation of ALL cells. Furthermore, they may either increase or decrease cytotoxicity of anticancer drugs. This heterogeneous response to growth factors concerning induction of proliferation and modulation of drug resistance should be taken into account in their clinical use.

Details

ISSN :
09395555
Volume :
78
Database :
OpenAIRE
Journal :
Annals of Hematology
Accession number :
edsair.doi.dedup.....f81b5c4193e84bff844a9380946a9bdb
Full Text :
https://doi.org/10.1007/s002770050495