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Skin Delivery and in Vitro Biological Evaluation of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles for Skin Disorder Therapies
- Source :
- Molecules, Vol 21, Iss 1, p 116 (2016), Scopus, Repositório Institucional da UNESP, Universidade Estadual Paulista (UNESP), instacron:UNESP, Molecules; Volume 21; Issue 1; Pages: 116, Molecules
- Publication Year :
- 2016
- Publisher :
- MDPI AG, 2016.
-
Abstract
- Made available in DSpace on 2018-12-11T17:08:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-01-01 The aim of this study was to evaluate the skin delivery and in vitro biological activity of trans-resveratrol (RES)-loaded solid lipid nanoparticles (SLNs). The SLNs were composed of stearic acid, poloxamer 407, soy phosphatidylcholine (SPC), an aqueous phase and 0.1% RES. The particle size, polydispersity index (PdI) and zeta potential were analyzed by dynamic light scattering (DLS). The SLNs were analyzed by scanning electron microscopy (SEM-FEG) and differential scanning calorimetry (DSC). In vitro RES-SLN skin permeation/retention assays were conducted, and their tyrosinase inhibitory activity was evaluated. An MTT reduction assay was performed on HaCat keratinocytes to determine in vitro cytotoxicity. The formulations had average diameter lower than 200 nm, the addition of SPC promoted increases in PdI in the RES-SLNs, but decreases PdI in the RES-free SLNs and the formulations exhibited zeta potentials smaller than -3 mV. The DSC analysis of the SLNs showed no endothermic peak attributable to RES. Microscopic analysis suggests that the materials formed had nanometric size distribution. Up to 45% of the RES permeated through the skin after 24 h. The RES-loaded SLNs were more effective than kojic acid at inhibiting tyrosinase and proved to be non-toxic in HaCat keratinocytes. The results suggest that the investigated RES-loaded SLNs have potential use in skin disorder therapies. Departamento de Fármacos E Medicamentos Faculdade de Ciências Farmacêuticas UNESP-Universidade Estadual Paulista, Campus Araraquara Centro de Ciências Biológicas E da Saúde Universidade Tiradentes Faculdade de Engenharia Química Universidade Estadual de Campinas Departamento de Fármacos E Medicamentos Faculdade de Ciências Farmacêuticas UNESP-Universidade Estadual Paulista, Campus Araraquara
- Subjects :
- Trans-resveratrol
Chemistry, Pharmaceutical
Tyrosinase
Pharmaceutical Science
solid lipid nanoparticle
Skin disorders
02 engineering and technology
Analytical Chemistry
chemistry.chemical_compound
Drug Delivery Systems
0302 clinical medicine
Stilbenes
Drug Discovery
Zeta potential
drug delivery system
Skin
Drug Carriers
Calorimetry, Differential Scanning
integumentary system
Permeation
021001 nanoscience & nanotechnology
Lipids
skin disorders
Biochemistry
Chemistry (miscellaneous)
030220 oncology & carcinogenesis
Molecular Medicine
permeation
Solid lipid nanoparticle
0210 nano-technology
medicine.drug
Drug delivery system
tyrosinase
Skin Diseases
Article
Permeability
Cell Line
lcsh:QD241-441
03 medical and health sciences
Dynamic light scattering
lcsh:Organic chemistry
Phosphatidylcholine
medicine
Humans
Particle Size
Physical and Theoretical Chemistry
Chromatography
Organic Chemistry
trans-resveratrol
body regions
HaCaT
chemistry
Resveratrol
Poloxamer 407
Nanoparticles
Kojic acid
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Volume :
- 21
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....f8740f7b9eba2b227de9d05c0b158ea1