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MRI monitoring of transplanted neonatal porcine islets labeled with polyvinylpyrrolidoneācoated superparamagnetic iron oxide nanoparticles in a mouse model
- Source :
- Xenotransplantation. 29
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- Islet transplantation is a potential treatment option for certain patients with type 1 diabetes; however, it still faces barriers to widespread use, including the lack of tools to monitor islet grafts post-transplantation. This study investigates whether labeling neonatal porcine islets (NPI) with polyvinylpyrrolidone-coated superparamagnetic iron oxide nanoparticles (PVP-SPIO) affects their function, and whether this nanoparticle can be utilized to monitor NPI xenografts with magnetic resonance imaging (MRI) in a mouse model. In vitro, PVP-SPIO-labeled NPI in an agarose gel was visualized clearly by MRI. PVP-SPIO-labeled islets were then transplanted under the kidney capsules of immunodeficient nondiabetic and diabetic mice. All diabetic mice that received transplantation of PVP-SPIO-labeled islets reached normoglycemia. Grafts appeared as hypo-intense areas on MRI and were distinguishable from the surrounding tissues. Following injection of spleen cells from immunocompetent mice, normoglycemic recipient mice became diabetic and islet grafts showed an increase in volume, accompanied by a mixed signal on MRI. Overall, this study demonstrates that PVP-SPIO did not affect the function of NPI that PVP-SPIO-labeled islets were easily seen on MRI, and changes in MRI signals following rejection suggest a potential use of PVP-SPIO-labeled islets to monitor graft viability.
- Subjects :
- endocrine system
Pathology
medicine.medical_specialty
Swine
Xenotransplantation
medicine.medical_treatment
Transplantation, Heterologous
Immunology
Islets of Langerhans Transplantation
Spleen
Diabetes Mellitus, Experimental
Islets of Langerhans
Mice
medicine
Animals
Humans
Transplantation
Kidney
geography
Type 1 diabetes
geography.geographical_feature_category
medicine.diagnostic_test
business.industry
technology, industry, and agriculture
Povidone
Magnetic resonance imaging
Islet
medicine.disease
Magnetic Resonance Imaging
In vitro
medicine.anatomical_structure
Magnetic Iron Oxide Nanoparticles
business
Subjects
Details
- ISSN :
- 13993089 and 0908665X
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- Xenotransplantation
- Accession number :
- edsair.doi.dedup.....f890d0ba2a93bfc2e2ada22a3a9c1a67