Back to Search
Start Over
Birth of piglets from in vitro-produced porcine blastocysts vitrified and warmed in a chemically defined medium
- Source :
- Theriogenology. 84(8)
- Publication Year :
- 2015
-
Abstract
- We examined the effect of different embryo developmental stages, culture periods, and media on the cryotolerance of in vitro-produced porcine blastocysts. All media used for in vitro production, vitrification, and warming were chemically defined. When in vitro-produced embryos at the blastocyst and expanded blastocyst stages were vitrified using the Cryotop method on Day 5 or 6 (Day 0 = the day of IVF), the survival rate and hatching rate of expanded blastocysts after warming were higher than those of blastocysts. The viability after vitrification and warming of Day-6 embryos cultured in porcine blastocyst medium from Day 5 were higher than that of embryos cultured in porcine zygote medium 5. On the other hand, there were no significant differences on the cryotolerance between Day-5 embryos cultured in porcine zygote medium 5 and those replaced with porcine blastocyst medium on Day 4. There were no significant differences in viability between the embryonic ages of 5 and 6 days after vitrification and warming. When expanded blastocysts vitrified on Day 5 or 6 were surgically transferred to recipient gilts, all three recipients became pregnant in the Day-5 group, whereas only one out of three recipients became pregnant in the Day-6 group. These results indicate that the cryotolerance of porcine in vitro-produced blastocysts after vitrification appears to depend on the embryonic stage, culture period, and medium.
- Subjects :
- medicine.medical_specialty
Time Factors
Swine
Embryonic Development
Fertilization in Vitro
Biology
Cryopreservation
Andrology
Embryo Culture Techniques
Food Animals
Pregnancy
medicine
Animals
Vitrification
Blastocyst
Small Animals
reproductive and urinary physiology
Zygote
urogenital system
Equine
Obstetrics
Embryogenesis
Embryo
Embryo Transfer
Embryo transfer
Culture Media
Chemically defined medium
medicine.anatomical_structure
embryonic structures
Animal Science and Zoology
Female
Subjects
Details
- ISSN :
- 18793231
- Volume :
- 84
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Theriogenology
- Accession number :
- edsair.doi.dedup.....f8a4ebee62c9587a83d73d975f3e6d23