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Haplotypes and Sequence Variation in the Ovine Adiponectin Gene (ADIPOQ)

Haplotypes and Sequence Variation in the Ovine Adiponectin Gene (ADIPOQ)

Authors :
Huitong Zhou
Jiang Hu
Jon G.H. Hickford
Yuzhu Luo
Qingming An
Source :
Genes, Genes, Vol 6, Iss 4, Pp 1230-1241 (2015), Volume 6, Issue 4, Pages 1230-1241
Publication Year :
2015
Publisher :
MDPI, 2015.

Abstract

The adiponectin gene (ADIPOQ) plays an important role in energy homeostasis. In this study five separate regions (regions 1 to 5) of ovine ADIPOQ were analysed using PCR-SSCP. Four different PCR-SSCP patterns (A1-D1, A2-D2) were detected in region-1 and region-2, respectively, with seven and six SNPs being revealed. In region-3, three different patterns (A3-C3) and three SNPs were observed. Two patterns (A4-B4, A5-B5) and two and one SNPs were observed in region-4 and region-5, respectively. In total, nineteen SNPs were detected, with five of them in the coding region and two (c.46T/C and c.515G/A) putatively resulting in amino acid changes (p.Tyr16His and p.Lys172Arg). In region-1, -2 and -3 of 316 sheep from eight New Zealand breeds, variants A1, A2 and A3 were the most common, although variant frequencies differed in the eight breeds. Across region-1 and region-3, nine haplotypes were identified and haplotypes A1-A3, A1-C3, B1-A3 and B1-C3 were most common. These results indicate that the ADIPOQ gene is polymorphic and suggest that further analysis is required to see if the variation in the gene is associated with animal production traits.

Details

Language :
English
ISSN :
20734425
Volume :
6
Issue :
4
Database :
OpenAIRE
Journal :
Genes
Accession number :
edsair.doi.dedup.....f8eff2ea251d32f2a20c493d739171cd