Back to Search
Start Over
γ-Carboxymuconolactone decarboxylase: a novel cell cycle-related basal body protein in the early branching eukaryote Trichomonas vaginalis
- Source :
- Parasites & Vectors, Vol 10, Iss 1, Pp 1-11 (2017), Parasites & Vectors
- Publication Year :
- 2017
- Publisher :
- BMC, 2017.
-
Abstract
- Background γ-Carboxymuconolactone decarboxylase (CMD) participates in the β-ketoadipate pathway, which catalyzes aromatic compounds to produce acetyl- or succinyl-CoA, in prokaryotes and yeast. Our previous study demonstrated that expression of a CMD homologue that contains two signatures (dualCMD) is negatively regulated by iron in Trichomonas vaginalis. However, we were not able to identify the components of the β-ketoadipate pathway in the parasite’s genome. These observations prompted us to investigate the biological functions of this novel CMD homologue in T. vaginalis. Methods The specific anti-TvCMD1 antibody was generated, and the expression of TvCMD1 in T. vaginalis cultured under iron-rich and iron-deficient were evaluated. Phylogenetic, metabolomic and substrate induction (protocatechuate and benzoate) analysis were conducted to clarify the function of dualCMD in trichomonad cells. Subcellular localization of TvCMD1 was observed by confocal microscopy. The cell cycle-related role of TvCMD1 was assessed by treating cells with G2/M inhibitor nocodazole. Results We confirmed that T. vaginalis is not able to catabolize the aromatic compounds benzoate and protocatechuate, which are known substrates of the β-ketoadipate pathway. Using immunofluorescence microscopy, we found that TvCMD1 is spatially associated with the basal body, a part of the cytoskeletal organizing center in T. vaginalis. TvCMD1 accumulated upon treatment with the G2/M inhibitor nocodazole. Additionally, TvCMD1 was expressed and transported to/from the basal body during cytokinesis, suggesting that TvCMD1 plays a role in cell division. Conclusion We demonstrated that TvCMD1 is unlikely to participate in the β-ketoadipate pathway and demonstrated that it is a novel basal body-localizing (associated) protein. This model sheds light on the importance of genes that are acquired laterally in the coevolution of ancient protists, which surprisingly functions in cell cycle regulation of T. vaginalis. Electronic supplementary material The online version of this article (10.1186/s13071-017-2381-4) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
Carboxy-Lyases
Iron
030106 microbiology
Cell
Protozoan Proteins
Biology
Cell cycle
medicine.disease_cause
Benzoates
lcsh:Infectious and parasitic diseases
03 medical and health sciences
chemistry.chemical_compound
medicine
Trichomonas vaginalis
Basal body
lcsh:RC109-216
Gene
Research
Iron deficiency
γ-carboxymuconolactone decarboxylase
biology.organism_classification
Basal Bodies
Lateral gene transfer
Cell biology
Nocodazole
030104 developmental biology
Infectious Diseases
medicine.anatomical_structure
Biochemistry
chemistry
Parasitology
Eukaryote
Cytokinesis
Subjects
Details
- Language :
- English
- ISSN :
- 17563305
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Parasites & Vectors
- Accession number :
- edsair.doi.dedup.....f9442003b62d664364589d01d8315635