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Loss of nuclear UBE3A activity is the predominant cause of Angelman syndrome in individuals carrying UBE3A missense mutations
- Source :
- Human Molecular Genetics, Human Molecular Genetics, 30(6), 430-442. Oxford University Press, Human molecular genetics, 30(6), 430-442. Oxford University Press
- Publication Year :
- 2021
-
Abstract
- Angelman syndrome (AS) is a severe neurodevelopmental disorder caused by deletion (~75%) or mutation (~10%) of the ubiquitin E3 ligase A (UBE3A) gene, which encodes a HECT type E3 ubiquitin protein ligase. Although the critical substrates of UBE3A are unknown, previous studies have suggested a critical role of nuclear UBE3A in AS pathophysiology. Here, we investigated to what extent UBE3A missense mutations disrupt UBE3A subcellular localization as well as catalytic activity, stability and protein folding. Our functional screen of 31 UBE3A missense mutants revealed that UBE3A mislocalization is the predominant cause of UBE3A dysfunction, accounting for 55% of the UBE3A mutations tested. The second major cause (29%) is a loss of E3-ubiquitin ligase activity, as assessed in an Escherichia coli in vivo ubiquitination assay. Mutations affecting catalytic activity are found not only in the catalytic HECT domain, but also in the N-terminal half of UBE3A, suggesting an important contribution of this N-terminal region to its catalytic potential. Together, our results show that loss of nuclear UBE3A E3 ligase activity is the predominant cause of UBE3A-linked AS. Moreover, our functional analysis screen allows rapid assessment of the pathogenicity of novel UBE3A missense variants which will be of particular importance when treatments for AS become available.
- Subjects :
- AcademicSubjects/SCI01140
HECT domain
congenital, hereditary, and neonatal diseases and abnormalities
Ubiquitin-Protein Ligases
Mutation, Missense
Saccharomyces cerevisiae
medicine.disease_cause
Mice
03 medical and health sciences
0302 clinical medicine
Angelman syndrome
Escherichia coli
Genetics
medicine
UBE3A
Animals
Humans
Missense mutation
Ligase activity
Molecular Biology
Genetics (clinical)
030304 developmental biology
Cell Nucleus
Neurons
chemistry.chemical_classification
0303 health sciences
DNA ligase
Mutation
biology
Ubiquitination
General Medicine
medicine.disease
Ubiquitin ligase
HEK293 Cells
chemistry
biology.protein
General Article
Angelman Syndrome
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 09646906
- Volume :
- 30
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Human molecular genetics
- Accession number :
- edsair.doi.dedup.....f9718602a9b38a7e220c2b1b5f796291