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Cisplatin upregulates MSH2 expression by reducing miR-21 to inhibit A549 cell growth
- Source :
- Biomedicine & Pharmacotherapy. 67:97-102
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- miR-21 can act as an oncogene. MSH2 has been reported that it involved in the DNA mismatch repair (MMR) system and overexpression of MSH2 can induce cell apoptosis. We predicted that MSH2-3'-untranslated region (3'-UTR) was targeted by miR-21 using microRNA analysis softwares. To further explore the roles of miR-21 and MSH2 in A549 cells, we constructed pcDNA-GFP-msh-UTR vector (including MSH2-3'-UTR) to transfect A549 cells with miR-21, GFP positive cells were estimated under a fluorescence microscopy and by flow cytometry. We found miR-21 could obviously downregulate the expression of MSH2, which was further proved by western blotting. Moreover, we treated A549 cells with cisplatin and found that cisplatin could inhibit A549 cell growth in vitro and in vivo. We also found that cisplatin could downregulate miR-21 expression, while increase MSH2 expression in A549 cells. Our results demonstrated that cisplatin could upregulate the expression of MSH2 through downregulating miR-21 to inhibit A549 cell proliferation, which provides new gene targets for drug design or cancer therary.
- Subjects :
- congenital, hereditary, and neonatal diseases and abnormalities
Down-Regulation
Cell Growth Processes
Biology
Article
Flow cytometry
Cell Line, Tumor
microRNA
Gene expression
medicine
Humans
3' Untranslated Regions
Pharmacology
A549 cell
Cisplatin
Oncogene
medicine.diagnostic_test
nutritional and metabolic diseases
General Medicine
Transfection
digestive system diseases
Up-Regulation
Gene Expression Regulation, Neoplastic
MicroRNAs
MutS Homolog 2 Protein
Apoptosis
Cancer research
medicine.drug
Subjects
Details
- ISSN :
- 07533322
- Volume :
- 67
- Database :
- OpenAIRE
- Journal :
- Biomedicine & Pharmacotherapy
- Accession number :
- edsair.doi.dedup.....f98c58a8a38f8b6afefb8590d69b7e13