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Use of a white light supercontinuum laser for confocal interference-reflection microscopy
- Source :
- Journal of Microscopy
- Publication Year :
- 2012
- Publisher :
- Blackwell Publishing Ltd, 2012.
-
Abstract
- Shortly after its development, the white light supercontinuum laser was applied to confocal scanning microscopy as a more versatile substitute for the multiple monochromatic lasers normally used for the excitation of fluorescence. This light source is now available coupled to commercial confocal fluorescence microscopes. We have evaluated a supercontinuum laser as a source for a different purpose: confocal interferometric imaging of living cells and artificial models by interference reflection. We used light in the range 460–700 nm where this source provides a reasonably flat spectrum, and obtained images free from fringe artefacts caused by the longer coherence length of conventional lasers. We have also obtained images of cytoskeletal detail that is difficult to see with a monochromatic laser.
- Subjects :
- Histology
Materials science
Microscope
Light
Confocal
Physics::Optics
Fluorescent Antibody Technique
Confocal scanning microscopy
Pathology and Forensic Medicine
law.invention
Mice
Optics
Two-photon excitation microscopy
law
Animals
Confocal laser scanning microscopy
Microscopy, Interference
interference reflection microscopy
Microscopy, Confocal
business.industry
Lasers
Scanning confocal electron microscopy
Original Articles
3T3 Cells
Fibroblasts
Laser
Supercontinuum
Light sheet fluorescence microscopy
supercontinuum laser
Optoelectronics
business
Subjects
Details
- Language :
- English
- ISSN :
- 13652818 and 00222720
- Volume :
- 246
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of Microscopy
- Accession number :
- edsair.doi.dedup.....f98ea1fc748a5537612eebff7aa6ee40