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Structural Dynamics of Zika Virus NS2B-NS3 Protease Binding to Dipeptide Inhibitors

Authors :
Ming Wei Chen
Alvin W. Hung
Zhenzhen Zhang
CongBao Kang
Dahai Luo
Weiling Wang
Wint Wint Phoo
Ying Ru Loh
Shuang Liu
Yan Li
Thomas H. Keller
Source :
Structure. 25:1242-1250.e3
Publication Year :
2017
Publisher :
Elsevier BV, 2017.

Abstract

The NS2B-NS3 viral protease is an attractive drug target against Zika virus (ZIKV) due to its importance in viral replication and maturation. Here we report the crystal structure of protease in complex with a dipeptide inhibitor, Acyl-KR-aldehyde (compound 1). The aldehyde moiety forms a covalent bond with the catalytic Ser135 of NS3. The Arg and Lys residues in the inhibitor occupy the S1 and S2 sites of the protease, respectively. Nuclear magnetic resonance studies demonstrate that the complex is in the closed conformation in solution. The chemical environment of residues surrounding the active site is sensitive to the bound inhibitor as demonstrated by the comparison with two other non-covalent dipeptides, Acyl-K-Agmatine (compound 2) and Acyl-KR-COOH (compound 3). Removing the aldehyde moiety in 1 converts the binding mode from a slow to a fast exchange regime. The structural dynamics information obtained in this study will guide future drug discovery against ZIKV and other flaviviruses.

Details

ISSN :
09692126
Volume :
25
Database :
OpenAIRE
Journal :
Structure
Accession number :
edsair.doi.dedup.....f98fa51f1d08881cf2e6521064dcde7d
Full Text :
https://doi.org/10.1016/j.str.2017.06.006