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Circulating miRNA panel for prediction of acute graft-versus-host disease in lymphoma patients undergoing matched unrelated hematopoietic stem cell transplantation
- Source :
- Experimental Hematology. (7):624-634.e1
- Publisher :
- ISEH - International Society for Experimental Hematology. Published by Elsevier Inc.
-
Abstract
- Acute graft-versus-host disease (aGVHD) results in significant morbidity and mortality after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Noninvasive diagnostic and prognostic tests for aGVHD are currently lacking, but would be beneficial in predicting aGVHD and improving the safety of allo-HSCT. Circulating microRNAs exhibit marked stability and may serve as biomarkers in several clinical settings. Here, we evaluated the use of circulating microRNAs as predictive biomarkers of aGVHD in lymphoma patients after allo-HSCT from matched unrelated donors (MUDs). After receiving informed consent, we prospectively collected plasma samples from 24 lymphoma patients before and after unmanipulated MUD allo-HSCT; microRNAs were then isolated. Fourteen patients developed aGVHD symptoms at a median of 48 days (range: 32–90) post-transplantation. Two patients developed intestinal GVHD, eight cutaneous GVHD, and four multiorgan GVHD. The microRNA expression profile was examined using quantitative real-time polymerase chain reaction (qRT-PCR). MicroRNAs 194 and 518f were significantly upregulated in aGVHD samples compared with samples taken from non-aGVHD patients. Remarkably, these upregulated microRNAs could be detected before the onset of aGVHD. Pathway prediction analysis indicated that these microRNAs may regulate critical pathways involved in aGVHD pathogenesis. Considering the noninvasive characteristics of plasma sampling and the feasibility of detecting miRNAs after allo-HSCT using real-time polymerase chain reaction, our results indicate that circulating microRNAs have the potential to enable an earlier aGVHD diagnosis and might assist in individualizing therapeutic strategies after MUD allo-HSCT. Nevertheless, standardization of blood sampling and analysis protocols is mandatory for the introduction of miRNA profiling into routine clinical use.
- Subjects :
- 0301 basic medicine
Adult
Male
Cancer Research
Time Factors
Adolescent
Lymphoma
medicine.medical_treatment
Gene Expression
Graft vs Host Disease
Disease
Hematopoietic stem cell transplantation
Pathogenesis
03 medical and health sciences
Young Adult
immune system diseases
hemic and lymphatic diseases
microRNA
Genetics
medicine
Cluster Analysis
Humans
Transplantation, Homologous
Gene Regulatory Networks
Young adult
Molecular Biology
Aged
integumentary system
business.industry
Gene Expression Profiling
Hematopoietic Stem Cell Transplantation
Reproducibility of Results
Cell Biology
Hematology
Middle Aged
medicine.disease
Prognosis
Combined Modality Therapy
Circulating MicroRNA
MicroRNAs
030104 developmental biology
surgical procedures, operative
Immunology
Female
business
Biomarkers
Blood sampling
Subjects
Details
- Language :
- English
- ISSN :
- 0301472X
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Experimental Hematology
- Accession number :
- edsair.doi.dedup.....f990de5fef670b036ac8fced93ac936a
- Full Text :
- https://doi.org/10.1016/j.exphem.2016.03.005