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Graphene-Based Sensing Platform for On-Chip Ochratoxin A Detection
- Source :
- Toxins, Toxins, Vol 11, Iss 10, p 550 (2019)
- Publication Year :
- 2019
-
Abstract
- In this work, we report an on-chip aptasensor for ochratoxin A (OTA) toxin detection that is based on a graphene field-effect transistor (GFET). Graphene-based devices are fabricated via large-scale technology, allowing for upscaling the sensor fabrication and lowering the device cost. The sensor assembly was performed through covalent bonding of graphene’s surface with an aptamer specifically sensitive towards OTA. The results demonstrate fast (within 5 min) response to OTA exposure with a linear range of detection between 4 ng/mL and 10 pg/mL, with a detection limit of 4 pg/mL. The regeneration time constant of the sensor was found to be rather small, only 5.6 s, meaning fast sensor regeneration for multiple usages. The high reproducibility of the sensing response was demonstrated via using several recycling procedures as well as various GFETs. The applicability of the aptasensor to real samples was demonstrated for spiked red wine samples with recovery of about 105% for a 100 pM OTA concentration; the selectivity of the sensor was also confirmed via addition of another toxin, zearalenone. The developed platform opens the way for multiplex sensing of different toxins using an on-chip array of graphene sensors.
- Subjects :
- Ochratoxin A
Materials science
Health, Toxicology and Mutagenesis
Aptamer
lcsh:Medicine
02 engineering and technology
Biosensing Techniques
Toxicology
01 natural sciences
law.invention
chemistry.chemical_compound
on-chip
law
Limit of Detection
sensor
Multiplex
Detection limit
Reproducibility
business.industry
Graphene
Communication
lcsh:R
010401 analytical chemistry
Transistor
graphene
aptamer
Aptamers, Nucleotide
021001 nanoscience & nanotechnology
Ochratoxins
0104 chemical sciences
Linear range
chemistry
Optoelectronics
transistor
Graphite
0210 nano-technology
business
ochratoxin A
Subjects
Details
- ISSN :
- 20726651
- Volume :
- 11
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Toxins
- Accession number :
- edsair.doi.dedup.....f994a132dc29b75edc8e30f93e346fc8