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Chiral analysis of methadone in plasma by high-performance liquid chromatography
- Source :
- Journal of chromatography. 570(1)
- Publication Year :
- 1991
-
Abstract
- A method for the chiral high-performance liquid chromatographic analysis of methadone in plasma has been developed. The method employed organic solvent extraction, enantiomeric separation on a Chiral AGP column, and ultraviolet absorption detection at 212 nm. The intra-day variation in the quantification of methadone enantiomers was less than 9% at the 100 ng/ml level, and the values obtained correlated well with those from a gas chromatographic-mass spectrometric method. Results from patients indicate inter- and intra-individual differences in the ratio between l- and d-methadone in plasma during therapy with racemic methadone. In one patient, a higher level of d-methadone in plasma was caused by both faster elimination and lower bioavailability of l-methadone.
- Subjects :
- Chromatography
medicine.diagnostic_test
Chemistry
Extraction (chemistry)
Analytical chemistry
Stereoisomerism
General Chemistry
High-performance liquid chromatography
Gas Chromatography-Mass Spectrometry
Bioavailability
Spectrophotometry
medicine
Humans
Spectrophotometry, Ultraviolet
Enantiomer
Gas chromatography–mass spectrometry
Chirality (chemistry)
Chromatography, High Pressure Liquid
Methadone
medicine.drug
Subjects
Details
- Volume :
- 570
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of chromatography
- Accession number :
- edsair.doi.dedup.....f9964ee5ce2ade08466a85fc799ead86