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Diacerein protects rats with liver ischemia/reperfusion damage: Down-regulation of TLR4/ NFκ-B signaling pathway
- Source :
- Biomedicine & Pharmacotherapy, Vol 134, Iss, Pp 111063-(2021)
- Publication Year :
- 2021
- Publisher :
- Elsevier, 2021.
-
Abstract
- Purpose Liver ischemia-reperfusion (I/R) injury is an inescapable problem. Diacerein, a chondro-protective drug, has antioxidant and anti-inflammatory effects. Its effect on liver I/R injury has not yet been fully clarified. Therefore, the current study aimed to detect its hepatic protective effect with the explanation of possible underlying mechanisms. Methods Adult male albino rats were assigned to 4 groups: sham group, diacerein pretreated sham group, I/R non-treated group, and I/R diacerein pretreated group. Serum liver enzymes, hepatic tissue oxidative stress parameters, inflammatory biomarkers mainly Toll-like receptors-4 (TLR4), and liver fatty acid binding protein (L-FABP) levels were determined. Histopathological examination of liver tissues and immunohistochemical studies of heat shock protein 70, nuclear factor-kappa B, and Cluster of Differentiation 68 were also done. Results Diacerein pretreatment has the ability to restore the hepatic I/R damaging effect, proved by the reduction of serum liver enzymes, the decrease of the oxidative stress and hepatic inflammation via down-regulation of TLR4/ NFκ-B signaling pathway together with the restoration of L-FABP level and improvement of the histopathological and immunohistochemical study findings in the hepatic tissue. Conclusion These results suggested the hepatoprotective effect of diacerein relies on its antioxidant and anti-inflammatory effects reducing TLR4/ NFκ-B signaling pathway.
- Subjects :
- 0301 basic medicine
Male
Diacerein
Antioxidant
medicine.medical_treatment
Anti-Inflammatory Agents
Down-Regulation
Vascular Cell Adhesion Molecule-1
Anthraquinones
RM1-950
Pharmacology
medicine.disease_cause
Fatty Acid-Binding Proteins
Protective Agents
Antioxidants
03 medical and health sciences
L-FABP
0302 clinical medicine
Downregulation and upregulation
Liver ischemia-reperfusion
Inflammatory pathway
Medicine
Animals
Cluster of differentiation
business.industry
Tumor Necrosis Factor-alpha
Liver Diseases
NF-kappa B
Alanine Transaminase
General Medicine
Hsp70
Rats
Toll-Like Receptor 4
Oxidative Stress
030104 developmental biology
Liver
030220 oncology & carcinogenesis
Reperfusion Injury
TLR4
Therapeutics. Pharmacology
Signal transduction
business
Oxidative stress
medicine.drug
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 07533322
- Volume :
- 134
- Database :
- OpenAIRE
- Journal :
- Biomedicine & Pharmacotherapy
- Accession number :
- edsair.doi.dedup.....f9a506c382d66bfdd4908ff0f18be985