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Cell detection by surface imprinted polymers SIPs: A study to unravel the recognition mechanisms
- Source :
- Sensors and Actuators B-Chemical, 255, 907-917. Elsevier Science
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Previous studies have shown that selective synthetic cell receptors can be produced by cell imprinting on polymer layers. However, knowledge on the fundamental detection mechanisms remains limited. In this article, while using yeast cells (Saccharomyces cerevisiae) as model cells, the factors influencing cellular recognition by surface-imprinted polymers (SIPs) are studied by means of spectroscopic and microscopy techniques and a transducer platform based on interfacial thermal transport, the so-called heat-transfer method (HTM). These analyses indicate that cell imprinting creates selective binding sites on the surface of the SIP layer in the form of binding cavities that match the cells in shape and size. Also, we show that phospholipid moieties are incorporated into the SIP cavities during imprinting, while membrane proteins do not seem to be transferred. More importantly, we demonstrate that the incorporated phospholipids significantly enhance cell adhesion to the SIP, and thus play a significant role in the cell-SIP binding mechanism. Furthermore, the hydrophobicity of the SIP layer was found to be considerably higher when compared with a non-imprinted polymer layer (NIP), an effect that could not be attributed to the presence of cavities on the surface of the SIP layer. Therefore, we suggest that the role of phospholipids in the SIP recognition mechanism is mediated by long range hydrophobic forces. ispartof: Sensors and Actuators B, Chemical vol:255 issue:1 pages:907-917 status: published
- Subjects :
- Engineering
PROTEINS
membrane proteins
Nanotechnology
02 engineering and technology
ADHESION
FILMS
010402 general chemistry
01 natural sciences
Thermal transport
Membrane proteins
Materials Chemistry
CANCER-CELLS
Electrical and Electronic Engineering
biomimetic sensors
Instrumentation
phospholipids
Phospholipids
HYDROPHOBICITY
PATHOGENS
Cell detection
business.industry
THERMAL TRANSPORT
Metals and Alloys
Cell adhesion
cell adhesion
Surface-imprinted polymers
021001 nanoscience & nanotechnology
Condensed Matter Physics
0104 chemical sciences
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
surface-imprinted polymers
FTIR
Synthetic Receptors
SYNTHETIC RECEPTORS
Biomimetic sensors
0210 nano-technology
business
Subjects
Details
- ISSN :
- 09254005
- Volume :
- 255
- Database :
- OpenAIRE
- Journal :
- Sensors and Actuators B: Chemical
- Accession number :
- edsair.doi.dedup.....fa13f84f0a2b6905f39dc28858a11f00