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Infection and Inflammation in Skeletal Muscle fromNonhuman Primates Infected with Different Genospecies of theLyme Disease SpirocheteBorreliaburgdorferi
- Source :
- Infection and Immunity. 71:7087-7098
- Publication Year :
- 2003
- Publisher :
- American Society for Microbiology, 2003.
-
Abstract
- Lyme borreliosis is a multisystemic disease caused by various genospecies of the spirocheteBorrelia burgdorferi. To investigate muscle involvement in the nonhuman primate (NHP) model of Lyme disease, 16 adultMacaca mulattaanimals inoculated with strain N40 ofB. burgdorferisensu strictu by syringe or by tick bite or with strain Pbi ofB. burgdorferigenospeciesgariniiby syringe were studied. Animals were necropsied while immunosuppressed on day 50 (two animals each inoculated withB.burgdorferiN40 by syringe and withB.gariniiPbi by syringe) or on day 90, 40 days after immunosuppression had been discontinued (four animals each inoculated with strain N40 by syringe, with strain N40 by tick bite, and with strain Pbi by syringe). Skeletal muscles removed at necropsy were studied by (i) microscopic examination of hematoxylin-eosin-stained sections for inflammation and tissue injury; (ii) immunohistochemical and digital image analyses for antibody and complement deposition and cellular inflammation; (iii) Western blot densitometry for the presence of antibodies; and (iv) reverse transcription-PCR for measurement of the spirochetal load or C1q (the first component of the complement cascade) synthesis. The results showed that N40 was more infectious for NHPs than Pbi. NHPs inoculated with N40 but not with Pbi developed myositis. The inflammation in skeletal muscle was more severe in NHPs inoculated with N40 by syringe than in those inoculated by tick bite. The predominant cells in the inflammatory infiltrate were T cells and plasma cells. The deposition of antibody and complement in inflamed muscles from N40-inoculated NHPs was significantly higher than that in Pbi-inoculated NHPs. The spirochetal load was very high in the two N40-inoculated NHPs examined while they were immunosuppressed but decreased to minimal levels in the NHPs when immunocompetence was restored. We conclude that myositis can be a prominent feature of Lyme borreliosis depending on the infecting organism and host immune status.
- Subjects :
- Male
Immunology
Anti-Inflammatory Agents
Spirochaetaceae
Microbiology
Dexamethasone
Lyme disease
Image Processing, Computer-Assisted
otorhinolaryngologic diseases
medicine
Animals
Humans
Bites and Stings
Borrelia burgdorferi
Muscle, Skeletal
Myositis
Immunosuppression Therapy
Inflammation
Lyme Disease
Ixodes
biology
Bacterial Infections
bacterial infections and mycoses
biology.organism_classification
medicine.disease
Antibodies, Bacterial
Immunohistochemistry
Macaca mulatta
Virology
Disease Models, Animal
Infectious Diseases
Needles
biology.protein
Lyme disease microbiology
Parasitology
Immunocompetence
Antibody
Subjects
Details
- ISSN :
- 10985522 and 00199567
- Volume :
- 71
- Database :
- OpenAIRE
- Journal :
- Infection and Immunity
- Accession number :
- edsair.doi.dedup.....fa154f8187f79a3f285f43408aead401
- Full Text :
- https://doi.org/10.1128/iai.71.12.7087-7098.2003