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Lack of an adrenal cortex inSf1mutant mice is compatible with the generation and differentiation of chromaffin cells
Lack of an adrenal cortex inSf1mutant mice is compatible with the generation and differentiation of chromaffin cells
- Source :
- Development. 132:4611-4619
- Publication Year :
- 2005
- Publisher :
- The Company of Biologists, 2005.
-
Abstract
- The diversification of neural-crest-derived sympathoadrenal (SA) progenitor cells into sympathetic neurons and neuroendocrine adrenal chromaffin cells was thought to be largely understood. In-vitro studies with isolated SA progenitor cells had suggested that chromaffin cell differentiation depends crucially on glucocorticoids provided by adrenal cortical cells. However, analysis of mice lacking the glucocorticoid receptor gene had revealed that adrenal chromaffin cells develop mostly normally in these mice. Alternative cues from the adrenal cortex that may promote chromaffin cell determination and differentiation have not been identified. We therefore investigated whether the chromaffin cell phenotype can develop in the absence of an adrenal cortex, using mice deficient for the nuclear orphan receptor steroidogenic factor-1 (SF1), which lack adrenal cortical cells and gonads. We show that in Sf1–/– mice typical chromaffin cells assemble correctly in the suprarenal region adjacent to the suprarenal sympathetic ganglion. The cells display most features of chromaffin cells, including the typical large chromaffin granules. Sf1–/–chromaffin cells are numerically reduced by about 50% compared with the wild type at embryonic day (E) 13.5 and E17.5. This phenotype is not accounted for by reduced survival or cell proliferation beyond E12.5. However, already at E12.5 the `adrenal' region in Sf1–/– mice is occupied by fewer PHOX2B+ and TH+ SA cells as well as SOX10+ neural crest cells. Our results suggest that cortical cues are not essential for determining chromaffin cell fate, but may be required for proper migration of SA progenitors to and/or colonization of the adrenal anlage.
- Subjects :
- endocrine system
medicine.medical_specialty
Chromaffin Cells
Receptors, Cytoplasmic and Nuclear
Biology
Steroidogenic Factor 1
Mice
Glucocorticoid receptor
Internal medicine
medicine
Animals
Progenitor cell
Molecular Biology
Homeodomain Proteins
Mice, Knockout
SOXE Transcription Factors
Adrenal cortex
Cell growth
High Mobility Group Proteins
Gene Expression Regulation, Developmental
Neural crest
Cell Differentiation
Sympathetic ganglion
Embryonic stem cell
Neoplasm Proteins
Microscopy, Electron
medicine.anatomical_structure
Endocrinology
Mutation
Chromaffin cell
Adrenal Cortex
Transcription Factors
Developmental Biology
Subjects
Details
- ISSN :
- 14779129 and 09501991
- Volume :
- 132
- Database :
- OpenAIRE
- Journal :
- Development
- Accession number :
- edsair.doi.dedup.....fa24e01088f6d18d5287964971ddf2e3
- Full Text :
- https://doi.org/10.1242/dev.02052