Assessment of Local Mosquito Species Incriminates Aedes aegypti as the Potential Vector of Zika Virus in Australia

Background Within the last 10 years Zika virus (ZIKV) has caused unprecedented epidemics of human disease in the nations and territories of the western Pacific and South America, and continues to escalate in both endemic and non-endemic regions. We evaluated the vector competence of Australian mosquitoes for ZIKV to assess their potential role in virus transmission. Methodology/Principal Findings Mosquitoes were exposed to infectious blood meals containing the prototype African ZIKV strain. After 14 days incubation at 28°C and high relative humidity, infection, dissemination and transmission rates were assessed. Infection in Culex annulirostris and Cx. sitiens could not be detected. 8% of Cx. quinquefasciatus were infected, but the virus did not disseminate in this species. Despite having infection rates > 50%, Aedes notoscriptus and Ae. vigilax did not transmit ZIKV. In contrast, Ae. aegypti had infection and transmission rates of 57% and 27%, respectively. In susceptibility trials, the virus dose required to infect 50% (ID50) of Ae. aegypti was106.4 tissue culture infectious dose50 (TCID50)/mL. Additionally, a threshold viral load within the mosquito of at least 105.1 TCID50 equivalents/mL had to be reached before virus transmission occurred. Conclusions/Significance We confirmed Ae. aegypti to be the most likely mosquito vector of ZIKV in Australia, although the restricted distribution of this species will limit the receptive zone to northern Queensland where this species occurs. Importantly, the role in ZIKV transmission of Culex and other Aedes spp. tested will be negligible. Despite being the implicated vector, the relatively high ID50 and need for a high titer disseminated infection in Ae. aegypti suggest that high mosquito population densities will be required to facilitate epidemic ZIKV transmission among the currently immunologically naïve human population in Australia.
Author Summary Zika virus was first isolated in Uganda in 1947 and exists in a transmission cycle between mosquitoes and non-human primates or humans. Whilst most clinical infections result in a self-limiting febrile illness, Zika virus has recently been linked to neurological syndromes, such as Guillain-Barré syndrome and congenital birth defects. Since 2007, Zika virus has undergone a dramatic range expansion, causing epidemics in nations and territories of the western Pacific and South America. To assess the emergence and transmission risk of Zika virus emerging in Australia, we evaluated the ability of local mosquitoes to become infected with and transmit the prototype African Zika virus strain. In agreement with its substantiated role in Zika virus transmission overseas, Australian Aedes aegypti were shown to be competent vectors. Coupled with its anthropophilic feeding behavior, this species should be considered the primary potential Zika virus vector in Australia. Although other common Australian species, such as Ae. notoscriptus and Ae. vigilax, were readily infected, they did not transmit the virus. The species of Culex tested were either refractory to infection or had a low infection rate. We also demonstrated that the Zika virus dose necessary to infect Ae. aegypti was higher than virus levels reported in infected humans. Finally, a high threshold level of virus circulating through the mosquito body was required before Ae. aegypti transmitted the virus. These results suggest that an outbreak of Zika virus in Australia would require high mosquito population densities and a susceptible human population.