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Cytotoxic Potential of the Coelomic Fluid Extracted from the Sea Cucumber Holothuria tubulosa against Triple-Negative MDA-MB231 Breast Cancer Cells
- Source :
- Biology, Volume 8, Issue 4, Biology, Vol 8, Iss 4, p 76 (2019)
- Publication Year :
- 2019
- Publisher :
- MDPI AG, 2019.
-
Abstract
- Growing evidence has demonstrated that the extracts of different holothurian species exert beneficial effects on human health. Triple negative breast cancers (TNBC) are highly malignant tumors that present a poor prognosis due to the lack of effective targeted therapies. In the attempt to identify novel compounds that might counteract TNBC cell growth, we studied the effect of the exposure of the TNBC cell line MDA-MB231 to total and filtered aqueous extracts of the coelomic fluid obtained from the sea cucumber Holoturia tubulosa, a widespread species in the Mediterranean Sea. In particular, we examined cell viability and proliferative behaviour, cell cycle distribution, apoptosis, autophagy, and mitochondrial metabolic/cell redox state. The results obtained indicate that both total and fractionated extracts are potent inhibitors of TNBC cell viability and growth, acting through both an impairment of cell cycle progression and mitochondrial transmembrane potential and a stimulation of cellular autophagy, as demonstrated by the increase of the acidic vesicular organelles and of the intracellular protein markers beclin-1, and total LC3 and LC3-II upon early exposure to the preparations. Identification of the water-soluble bioactive component(s) present in the extract merit further investigation aiming to develop novel prevention and/or treatment agents efficacious against highly metastatic breast carcinomas.
- Subjects :
- 0301 basic medicine
autophagy
Cell
Settore BIO/05 - Zoologia
Biology
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
0302 clinical medicine
breast cancer
mitochondrial function
Organelle
medicine
Cytotoxic T cell
Viability assay
Holothuria tubulosa
Settore BIO/06 - Anatomia Comparata E Citologia
lcsh:QH301-705.5
cell viability
General Immunology and Microbiology
Autophagy
Cell cycle
biology.organism_classification
030104 developmental biology
medicine.anatomical_structure
lcsh:Biology (General)
Apoptosis
030220 oncology & carcinogenesis
Cancer research
cell cycle
General Agricultural and Biological Sciences
coelomic fluid
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Biology, Volume 8, Issue 4, Biology, Vol 8, Iss 4, p 76 (2019)
- Accession number :
- edsair.doi.dedup.....fa36d5c8b9625634ba647f97530d6b29