Screening of Bioactive Properties in Brown Algae from the Northwest Iberian Peninsula

Algae are an underexploited source of natural bioactive compounds in Western countries, so an increasing interest in the valorization of these marine organisms has emerged in recent years. In this work, the effect of extracting solvent on the extraction yield, phenolic content, antioxidant capacity, and antimicrobial activity of nine brown macroalgae species (Ascophyllum nodosum, Himanthalia elongata, Undaria pinnatifida, Pelvetia canaliculata, Saccharina latissima, Bifurcaria bifurcata, Laminaria ochroleuca, Sargassum muticum, and Fucus spiralis) was assessed. Total phenolic content (TPC) and the antioxidant properties of extracts by different assays: radical scavenging activity (DPPH-RSA) and ferric reducing antioxidant power (FRAP) were performed. The antimicrobial activity of extracts was studied against six different foodborne microorganisms: Staphylococcus aureus, Staphylococcus epidermidis, Bacillus cereus, Escherichia coli, Salmonella enteritidis, and Pseudomonas aeruginosa. The highest extraction yield was achieved in ethanolic extracts. However, the highest TPC and FRAP values were obtained on the ethyl acetate extracts, especially from A. nodosum. Concerning algal species, the highest TPC and FRAP values were found in A. nodosum, while the highest DPPH-RSA values were achieved in the hexane extracts of B. bifurcata. The antimicrobial activity of algal extracts varied according to the solvent and alga selected, suggesting the species- and solvent-dependent behavior of this property, with B. bifurcata extracts showing the highest results for a wide range of bacteria. Our results provide insight on the characterization of widespread brown algae in the coasts of the North-Western region of the Iberian Peninsula, reflecting multiple health-enhancing properties which may lead to their exploitation in food, pharmacological, and cosmetic industries. European Commission | Ref. UP4HEALTH (H2020-BBI-JTI-2019) n. 888003 Programa Iberoamericano de Ciencia y Tecnología | Ref. CYTED— AQUA-CIBUS, P317RT0003 European Commission | Ref. ERA-NET ERA-HDHL (H2020) (n 696295) Fundação para a Ciência e a Tecnologia | Ref. PTDC / OCE-ETA / 30240 / 2017 Fundação para a Ciência e a Tecnologia | Ref. POCI-01-0145-FEDER-030240 Fundação para a Ciência e a Tecnologia | Ref. UIDB / 50016/2020 Ministerio de Ciencia e Innovación | Ref. RYC-2017-22891 Xunta de Galicia | Ref. ED431F 2020/12 Xunta de Galicia | Ref. ED481A- 2019/295