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Positive Regulation of Decidualization by l-Type Amino Acid Transporter 1 (lat1) in Pregnant Mice
- Source :
- Nutrients; Volume 8; Issue 11; Pages: 704, Nutrients, Nutrients, Vol 8, Iss 11, p 704 (2016)
- Publication Year :
- 2016
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2016.
-
Abstract
- Amino acids have an important role in the pre and post implantation of placenta and embryo development. l-type amino-acid transporter 1 (lat1) is responsible for the transportation of large neutral amino acids and is mainly expressed in human fetal liver, placenta, brain, etc. This study is the first to investigate the expression of lat1 in the early pregnancy of mouse uteri and its role in the process of decidualization. Endometrial stromal cells of a mouse model were used to evaluate decidualization from Day 4–8 of pregnancy in vitro followed by lat1 knock down by small interfering RNA and by a competitive inhibitor of Leucine transport 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH). The effects of lat1 on decidualization in vivo were assessed by injecting BCH into the uterine horns. The mRNA and protein expressions of lat1 in the implantation sites were higher than that in the inter-implantation sites and were localized in the luminal and gland epithelium, stromal and decidual cells. Its increased expression (p < 0.05) was associated with artificial decidualization as well as activation of prl expression. Down-regulation of lat1 expression in these cells by siRNA and BCH inhibited the decidual progression in vitro. Inhibition of lat1 transportation by BCH controlled decidual progression in vivo also accompanied the down-regulation of prl, lat1 expression in the decidual area and embryo size on Day 8 of pregnancy. In conclusion, these results revealed that lat1 might play an important role in the decidual progression both in vitro and in vivo.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Small interfering RNA
Stromal cell
Amino Acid Transport System y+
Amino Acids, Cyclic
Down-Regulation
Embryonic Development
Gene Expression
lcsh:TX341-641
Gestational Age
Biology
Article
Andrology
03 medical and health sciences
Mice
l-amino acid transporter-1 (lat1)
In vivo
decidualization
Leucine
Pregnancy
l-amino+acid+transporter-1+%28lat1%29%22">">l-amino acid transporter-1 (lat1)
Placenta
Internal medicine
medicine
Decidua
Animals
Decidual cells
Embryo Implantation
RNA, Messenger
RNA, Small Interfering
Cells, Cultured
l-amino+acid+transporter-1+%28lat1%29%22">">l-amino acid transporter-1 (lat1)
stromal cell
pregnant mice
Messenger RNA
Nutrition and Dietetics
Leucine transport
Uterus
Decidualization
Amino Acid Transport System y+L
Biological Transport
030104 developmental biology
medicine.anatomical_structure
Endocrinology
Female
Stromal Cells
lcsh:Nutrition. Foods and food supply
Food Science
Subjects
Details
- Language :
- English
- ISSN :
- 20726643
- Database :
- OpenAIRE
- Journal :
- Nutrients; Volume 8; Issue 11; Pages: 704
- Accession number :
- edsair.doi.dedup.....fa8f3dd7e27cf1e90edc417117ab5fe6
- Full Text :
- https://doi.org/10.3390/nu8110704