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Staphylococcus enterotoxin B-induced T cells can efficaciously protect against type 1 diabetes in non-obese diabetic mice
- Source :
- Central-European Journal of Immunology
- Publication Year :
- 2015
- Publisher :
- Polish Society of Experimental and Clinical Immunology, 2015.
-
Abstract
- Type 1 diabetes (T1D), an autoimmune disease, can be protected against by natural killer T (NKT) cells. Several attempts demonstrate that NKT cells also can be produced by inducing with Staphylococcus enterotoxin B (SEB) in addition to its classical activated antigen α-galactosylceramide. Here, we examined a potential usage of SEB-induced T (SEB-T) cells for the treatment of T1D. We established the immunophenotypes of SEB-T cells via flow cytometry, and in consequence, enriched in CD8(+)NKT cells after SEB stimulated. A high level of transforming growth factor β (TGF-β), detected by RT-PCR and ELISA, was first observed to be expressed and secreted by these SEB-T cells. Mixed lymphocyte reactions indicated that SEB-T cells could not produce a response to mitogens and allogeneic lymphocyte, and can inhibit lymphocytes response to mitogens. In an animal model, our data indicated that infusion of SEB-T cells in non-obese diabetic mice was well tolerated and could ameliorate hyperglycemia and maintain the blood glucose nearly on normal level until sacrifice. Strikingly, infusion of SEB-T cells resulted in an increase in the serum TGF-β level. These data raise the possibility that SEB-T cells can protect against T1D, which is associated with NKT cells generated in these SEB-induced cells.
- Subjects :
- Original Paper
CD40
Staphylococcus enterotoxin B
adoptive transfer
biology
type 1 diabetes
ZAP70
Lymphocyte
Immunology
CD1
hemic and immune systems
chemical and pharmacologic phenomena
transforming growth factor-β
Natural killer T cell
biological factors
Interleukin 21
medicine.anatomical_structure
Antigen
biology.protein
Interleukin 12
medicine
Immunology and Allergy
CD8+ natural killer T
immunomodulatory
Subjects
Details
- Language :
- English
- ISSN :
- 16444124 and 14263912
- Volume :
- 40
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Central-European Journal of Immunology
- Accession number :
- edsair.doi.dedup.....faca34c6383b166306b7c1b52e65764b