Lack of correlation between bronchial late allergic reaction to Dermatophagoides pteronyssinus and in vitro immunoglobulin E reactivity to histamine-releasing factor derived from mononuclear cells

Background: Activity of immunoglobulin (Ig)E-dependent histamine-releasing factor (HRF) is dependent on the IgE molecules bound to the surface of basophils. Sera capable of passively sensitizing basophils to release histamine to HRF were designated IgE(+) sera. IgE(+) and HRF have been suggested to play a role in late allergic reaction (LAR). Objective: The working hypothesis was tested that IgE(+) induces a LAR. Further, activity of HRF produced by mononuclear cells (HRFmn) was compared with that of recombinant HRF p23. Methods: Atopic patients (n = 82) were bronchially provoked with Dermatophagoides pteronyssinus extract and the change in forced expiratory volume in I second was monitored. A LAR was defined as forced expiratory volume in 1 second as percentage of baseline