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Vibrio cholerae Typing Phage N4: Genome Sequence and Its Relatedness to T7 Viral Supergroup
- Source :
- Intervirology. 55:185-193
- Publication Year :
- 2011
- Publisher :
- S. Karger AG, 2011.
-
Abstract
- Objectives: In countries where cholera is endemic, Vibrio cholerae O1 bacteriophages have been detected in sewage water. These have been used to serve not only as strain markers, but also for the typing of V. cholerae strains. Vibriophage N4 (ATCC 51352-B1) occupies a unique position in the new phage-typing scheme and can infect a larger number of V. cholerae O1 biotype El Tor strains. Here we characterized the complete genome sequence of this typing vibriophage. Methods: The complete DNA sequence of the N4 genome was determined by using a shotgun sequencing approach. Results: Complete genome sequence explored that phage N4 is comprised of one circular, double-stranded chromosome of 38,497 bp with an overall GC content of 42.8%. A total of 47 open reading frames were identified and functions could be assigned to 30 of them. Further, a close relationship with another vibriophage, VP4, and the enterobacteriophage T7 could be established. DNA-DNA hybridization among V. cholerae O1 and O139 phages revealed homology among O1 vibriophages at their genomic level. Conclusions: This study indicates two evolutionary distinctive branches of the possible phylogenetic origin of O1 and O139 vibriophages and provides an unveiled collection of information on viral gene products of typing vibriophages.
- Subjects :
- Sequence analysis
Molecular Sequence Data
Genome, Viral
Biology
medicine.disease_cause
El Tor
Genome
Open Reading Frames
Virology
Bacteriophage N4
medicine
Cluster Analysis
Typing
Phylogeny
Comparative genomics
Genetics
Whole genome sequencing
Base Composition
Shotgun sequencing
Vibrio cholerae O1
Nucleic Acid Hybridization
Sequence Analysis, DNA
biology.organism_classification
Infectious Diseases
Vibrio cholerae
DNA, Viral
Subjects
Details
- ISSN :
- 14230100 and 03005526
- Volume :
- 55
- Database :
- OpenAIRE
- Journal :
- Intervirology
- Accession number :
- edsair.doi.dedup.....fb004d342a32a41af5319b182f5d1b0c