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Schwann cells genetically modified to express neurotrophins promote spiral ganglion neuron survival in vitro
- Source :
- Neuroscience. 152:821-828
- Publication Year :
- 2008
- Publisher :
- Elsevier BV, 2008.
-
Abstract
- The intracochlear infusion of neurotrophic factors via a mini-osmotic pump has been shown to prevent deafness-induced spiral ganglion neuron (SGN) degeneration; however, the use of pumps may increase the incidence of infection within the cochlea, making this technique unsuitable for neurotrophin administration in a clinical setting. Cell- and gene-based therapies are potential therapeutic options. This study investigated whether Schwann cells which were genetically modified to over-express the neurotrophins brain-derived neurotrophic factor (BDNF) or neurotrophin 3 (Ntf3, formerly NT-3) could support SGN survival in an in vitro model of deafness. Co-culture of either BDNF over-expressing Schwann cells or Ntf3 over-expressing Schwann cells with SGNs from early postnatal rats significantly enhanced neuronal survival in comparison to both control Schwann cells and conventional recombinant neurotrophin proteins. Transplantation of neurotrophin over-expressing Schwann cells into the cochlea may provide an alternative means of delivering neurotrophic factors to the deaf cochlea for therapeutic purposes.
- Subjects :
- Cell Survival
Hearing Loss, Sensorineural
Schwann cell
Cell Communication
Neurotrophin-3
Ciliary neurotrophic factor
Article
Neurotrophin 3
Neurotrophic factors
otorhinolaryngologic diseases
medicine
Animals
Nerve Growth Factors
Neurons, Afferent
Rats, Wistar
Cells, Cultured
Spiral ganglion
Brain-derived neurotrophic factor
biology
Brain-Derived Neurotrophic Factor
General Neuroscience
Genetic Therapy
Coculture Techniques
Rats
medicine.anatomical_structure
Animals, Newborn
Gene Expression Regulation
nervous system
Nerve Degeneration
biology.protein
Neuroglia
Schwann Cells
sense organs
Spiral Ganglion
Neuroscience
Neurotrophin
Subjects
Details
- ISSN :
- 03064522
- Volume :
- 152
- Database :
- OpenAIRE
- Journal :
- Neuroscience
- Accession number :
- edsair.doi.dedup.....fb039b1b7cfe2a03fbf08d9e8c06ee53