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Lysophosphatidic acid is a chemoattractant for Dictyostelium discoideum amoebae
Lysophosphatidic acid is a chemoattractant for Dictyostelium discoideum amoebae
- Source :
- Proceedings of the National Academy of Sciences of the United States of America. 90(5)
- Publication Year :
- 1993
-
Abstract
- The naturally occurring phospholipid lysophosphatidic acid (LPA) can induce a number of physiological responses in vertebrate cells, including platelet aggregation, smooth muscle contraction, and fibroblast proliferation. LPA is thought to activate a specific G-protein-coupled receptor, thereby triggering classic second messenger pathways such as stimulation of phospholipase C and inhibition of adenylate cyclase. Here we report that 1-oleoyl-LPA, at submicromolar concentrations, evokes a chemotactic response in amoebae of the cellular slime mold Dictyostelium discoideum. LPA-induced chemotaxis is specific in that other lysophospholipids, phosphatidic acid, and monoacylglycerol have no effect. We show that the response to LPA is not secondary to the accumulation of extracellular cAMP, a well-established chemoattractant for nutrient-starved D. discoideum. Compared with cAMP-induced chemotaxis, LPA-induced chemotaxis has a somewhat lower efficiency and is not accompanied by the characteristic cellular elongation and orientation along the gradient. These results indicate that LPA has a previously unsuspected role as a chemoattractant for D. discoideum and imply that its biological function as a "first messenger" is not restricted to vertebrate cells.
- Subjects :
- Multidisciplinary
biology
Phospholipase C
Chemotaxis
Lysophosphatidylcholines
Smooth muscle contraction
Phosphatidic acid
biology.organism_classification
Dictyostelium
Dictyostelium discoideum
Cell biology
chemistry.chemical_compound
chemistry
Biochemistry
Lysophosphatidic acid
Second messenger system
Cyclic AMP
Animals
lipids (amino acids, peptides, and proteins)
biological phenomena, cell phenomena, and immunity
Phospholipids
Signal Transduction
Research Article
Subjects
Details
- ISSN :
- 00278424
- Volume :
- 90
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Accession number :
- edsair.doi.dedup.....fb0a0dbfd4531d767d62fb8036a6e0f4