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Murine CD160, Ig-Like Receptor on NK Cells and NKT Cells, Recognizes Classical and Nonclassical MHC Class I and Regulates NK Cell Activation
- Source :
- The Journal of Immunology. 175:4426-4432
- Publication Year :
- 2005
- Publisher :
- The American Association of Immunologists, 2005.
-
Abstract
- Human and mouse NK cells use different families of receptors to recognize MHC class I (MHC I) on target cells. Although human NK cells express both Ig-like receptors and lectin-like receptors specific for MHC I, all the MHC I-specific receptors identified on mouse NK cells to date are lectin-like receptors, and no Ig-like receptors recognizing MHC I have been identified on mouse NK cells. In this study we report the first MHC I-specific Ig-like receptor on mouse NK cells, namely, murine CD160 (mCD160). The expression of mCD160 is restricted to a subset of NK cells, NK1.1+ T cells, and activated CD8+ T cells. The mCD160-Ig fusion protein binds to rat cell lines transfected with classical and nonclassical mouse MHC I, including CD1d. Furthermore, the level of mCD160 on NK1.1+ T cells is modulated by MHC I of the host. Overexpression of mCD160 in the mouse NK cell line KY-2 inhibits IFN-γ production induced by phorbol ester plus ionomycin, whereas it enhances IFN-γ production induced by NK1.1 cross-linking or incubation with dendritic cells. Cross-linking of mCD160 also inhibits anti-NK1.1-mediated stimulation of KY-2 cells. Anti-mCD160 mAb alone has no effect. Thus, mCD160, the first MHC I-specific Ig-like receptor on mouse NK cells, regulates NK cell activation both positively and negatively, depending on the stimulus.
- Subjects :
- Male
DNA, Complementary
T-Lymphocytes
Immunology
CD1
chemical and pharmacologic phenomena
GPI-Linked Proteins
Ligands
Lymphocyte Activation
Mice
Interleukin 21
Antigens, CD
MHC class I
Animals
Humans
Immunology and Allergy
Cytotoxic T cell
Receptors, Immunologic
Antigen-presenting cell
Cells, Cultured
Mice, Knockout
Mice, Inbred BALB C
Mice, Inbred C3H
biology
Chemistry
Janus kinase 3
Histocompatibility Antigens Class I
Membrane Proteins
Molecular biology
Rats, Inbred F344
Rats
Killer Cells, Natural
Mice, Inbred C57BL
CD1D
Interleukin 12
biology.protein
K562 Cells
Subjects
Details
- ISSN :
- 15506606 and 00221767
- Volume :
- 175
- Database :
- OpenAIRE
- Journal :
- The Journal of Immunology
- Accession number :
- edsair.doi.dedup.....fb1c95a85adcac38e7808bff906f8df2