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Solubilization steps of dark-adapted purple membrane by Triton X-100 A spectroscopic study
- Source :
- FEBS Letters. 305:249-253
- Publication Year :
- 1992
- Publisher :
- Wiley, 1992.
-
Abstract
- Ultraviolet-visible spectroscopy has been used to follow the solubilization of the dark-adapted purple membrane of Halobacterium halobium by Triton X-100. Turbidity of purple membrane fragments and absorbance of bacteriorhodopsin variations during continuous addition of detergent give solubilization profiles exhibiting several break points corresponding to different equilibrium stages of the solubilization process. The present method allows the determination of the detergent to protein + lipid ratio in mixed aggregates at the corresponding break points. It was concluded that, when performed systematically, this technique is a very convenient and powerful tool for the quantitative study of biomembrane-to-micelle transition.
- Subjects :
- Halobacterium salinarum
Light
Octoxynol
Detergents
Biophysics
Photoprotein
Analytical chemistry
Bacteriorhodopsin
Absorbance
Biochemistry
Polyethylene Glycols
Turbidity
chemistry.chemical_compound
Structural Biology
Spectrophotometry
Solubilization
Genetics
medicine
Halobacteriaceae
Solubility
Molecular Biology
Chromatography
Triton X-100
medicine.diagnostic_test
biology
Cell Biology
Darkness
Purple membrane
biology.organism_classification
Membrane
Models, Chemical
chemistry
Bacteriorhodopsins
biology.protein
Spectrophotometry, Ultraviolet
Subjects
Details
- ISSN :
- 00145793
- Volume :
- 305
- Database :
- OpenAIRE
- Journal :
- FEBS Letters
- Accession number :
- edsair.doi.dedup.....fb551110afe5cd1aa7d1bba1d5bf933f
- Full Text :
- https://doi.org/10.1016/0014-5793(92)80679-b