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A novel Escherichia coli O157:H7 clone causing a major hemolytic uremic syndrome outbreak in China
- Source :
- PLoS ONE, Vol 7, Iss 4, p e36144 (2012), PLoS ONE
- Publication Year :
- 2012
- Publisher :
- Public Library of Science (PLoS), 2012.
-
Abstract
- An Escherichia coli O157:H7 outbreak in China in 1999 caused 177 deaths due to hemolytic uremic syndrome. Sixteen outbreak associated isolates were found to belong to a new clone, sequence type 96 (ST96), based on multilocus sequence typing of 15 housekeeping genes. Whole genome sequencing of an outbreak isolate, Xuzhou21, showed that the isolate is phylogenetically closely related to the Japan 1996 outbreak isolate Sakai, both of which share the most recent common ancestor with the US outbreak isolate EDL933. The levels of IL-6 and IL-8 of peripheral blood mononuclear cells induced by Xuzhou21 and Sakai were significantly higher than that induced by EDL933. Xuzhou21 also induced a significantly higher level of IL-8 than Sakai while both induced similar levels of IL-6. The expression level of Shiga toxin 2 in Xuzhou21 induced by mitomycin C was 68.6 times of that under non-inducing conditions, twice of that induced in Sakai (32.7 times) and 15 times higher than that induced in EDL933 (4.5 times). Our study shows that ST96 is a novel clone and provided significant new insights into the evolution of virulence of E. coli O157:H7.
- Subjects :
- Bacterial Diseases
Male
Clone (cell biology)
Pathogenesis
medicine.disease_cause
Toxicology
Shiga Toxin 2
Disease Outbreaks
Japan
Escherichia Coli
Multidisciplinary
biology
Geography
Shiga toxin
Middle Aged
Housekeeping gene
Bacterial Pathogens
Infectious Diseases
Medical Microbiology
Cytokines
Medicine
Female
Research Article
Adult
China
Adolescent
Sequence analysis
Science
Toxic Agents
Molecular Sequence Data
Virulence
Escherichia coli O157
Microbiology
Young Adult
Age Distribution
medicine
Humans
Escherichia coli
Biology
Microbial Pathogens
Bacterial Evolution
Outbreak
Bacteriology
Sequence Analysis, DNA
Emerging Infectious Diseases
Genes, Bacterial
Microbial Evolution
Hemolytic-Uremic Syndrome
biology.protein
Multilocus sequence typing
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 7
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....fb5f3ef893e2282f52ca895aff944c31