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Ethidium bromide as a marker of mtDNA replication in living cells
- Publication Year :
- 2012
- Publisher :
- country:IT, 2012.
-
Abstract
- Mitochondrial DNA (mtDNA) in tumor cells was found to play an important role in maintaining the malignant phenotype. Using laser scanning confocal fluorescence microscopy (LSCFM) in a recent work, we reported a variable fluorescence intensity of ethidium bromide (EB) in mitochondria nucleoids of living carcinoma cells. Since when EB is bound to nucleic acids its fluorescence is intensified; a higher EB fluorescence intensity could reflect a higher DNA accessibility to EB, suggesting a higher mtDNA replication activity. To prove this hypothesis, in the present work we studied, by LSCFM, the EB fluorescence in mitochondria nucleoids of living neuroblastoma cells, a model system in which differentiation affects the level of mtDNA replication. A drastic decrease of fluorescence was observed after differentiation. To correlate EB fluorescence intensity to the mtDNA replication state, we evaluated the mtDNA nascent strands content by ligation-mediated real-time PCR, and we found a halved amount of replicating mtDNA molecules in differentiating cells. A similar result was obtained by BrdU incorporation. These results indicate that the low EB fluorescence of nucleoids in differentiated cells is correlated to a low content of replicating mtDNA, suggesting that EB may be used as a marker of mtDNA replication in living cells. © 2012 Society of Photo-Optical Instrumentation Engineers (SPIE).
- Subjects :
- DNA Replication
Mitochondrial DNA
Cellular differentiation
Fluorescent Dye
Biomedical Engineering
DNA, Mitochondrial
Biomaterials
Ligation-mediated PCR
chemistry.chemical_compound
Cell Line, Tumor
Ethidium
Fluorescence microscope
Image Processing, Computer-Assisted
Nucleoid
Humans
MtDNA nucleoids mtDNA replication
BrdU
Fluorescent Dyes
Ethidium bromide
Microscopy, Confocal
Human neuroblastoma cell
Electronic, Optical and Magnetic Material
DNA replication
Molecular biology
Biomaterial
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Mitochondria
Confocal fluorescence microscopy
chemistry
Bromodeoxyuridine
Microscopy, Fluorescence
DNA
Human
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....fb7cefdf6b6728ea4437060ff6d6d8b0