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Detection of cerebral tauopathy in P301L mice using high-resolution large-field multifocal illumination fluorescence microscopy
- Source :
- Biomed Opt Express
- Publication Year :
- 2020
- Publisher :
- Cold Spring Harbor Laboratory, 2020.
-
Abstract
- Current intravital microscopy techniques visualize tauopathy with high-resolution, but have a small field-of-view and depth-of-focus. Herein, we report a transcranial detection of tauopathy over the entire cortex of P301L tauopathy mice using large-field multifocal illumination (LMI) fluorescence microscopy technique and luminescent conjugated oligothiophenes. In vitro assays revealed that fluorescent ligand h-FTAA is optimal for in vivo tau imaging, which was confirmed by observing elevated probe retention in the cortex of P301L mice compared to non-transgenic littermates. Immunohistochemical staining further verified the specificity of h-FTAA to detect tauopathy in P301L mice. The new imaging platform can be leveraged in pre-clinical mechanistic studies of tau spreading and clearance as well as longitudinal monitoring of tau targeting therapeutics.
- Subjects :
- 10050 Institute of Pharmacology and Toxicology
High resolution
610 Medicine & health
3107 Atomic and Molecular Physics, and Optics
01 natural sciences
Article
170 Ethics
010309 optics
03 medical and health sciences
0302 clinical medicine
In vivo
Atomic and Molecular Physics
Cortex (anatomy)
0103 physical sciences
Microscopy
Fluorescence microscope
medicine
10237 Institute of Biomedical Engineering
030304 developmental biology
0303 health sciences
Chemistry
medicine.disease
Fluorescence
Atomic and Molecular Physics, and Optics
medicine.anatomical_structure
1305 Biotechnology
Biophysics
Immunohistochemistry
Tauopathy
and Optics
Preclinical imaging
030217 neurology & neurosurgery
Intravital microscopy
Biotechnology
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Biomed Opt Express
- Accession number :
- edsair.doi.dedup.....fb9aa68711316aa4134027362a25c483