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Subtyping of gliomas of various<scp>WHO</scp>grades by the application of immunohistochemistry
- Source :
- Histopathology
- Publication Year :
- 2013
- Publisher :
- Wiley, 2013.
-
Abstract
- Aims In 2010, four subtypes (classical, proneural, mesenchymal, and neural) of glioblastoma multiforme (GBM) were defined by molecular genetic analyses. The objective of this study was to assess whether gliomas, independently of the type and grade, could be subdivided into protein-based subtypes. Methods and results A tissue microarray (TMA) approach was applied to incorporate tissue samples of low-grade and high-grade gliomas into five TMAs. High expression levels of epidermal growth factor receptor (EGFR), CD44, c-MER proto-oncogene tyrosine kinase (MERTK), platelet-derived growth factor receptor α, p53, oligodendrocyte transcription factor 2 (OLIG2) and isocitrate dehydrogenase 1 with the R132H mutation were assessed using immunohistochemistry (IHC). Glioma could be subdivided into four subtypes by IHC. The majority of the low-grade gliomas were of the proneural subtype, i.e. high p53 expression (63% of grade II). The classical subtype, with high EGFR and low p53 expression, was most common in GBMs (39%), followed by the proneural (29%) and mesenchymal (with high CD44 and MERTK expression) (29%) subtypes, a frequency that is in line with previously published data based on molecular genetics. Conclusions Assessment of the expression of the five proteins EGFR, CD44, MERTK, p53 and OLIG2 is sufficient for subtyping gliomas, and can be recommended for implementation in clinical practice for both low-grade and high-grade gliomas.
- Subjects :
- Male
Pathology
Receptor, Platelet-Derived Growth Factor alpha
subtyping
Proto-Oncogene Mas
glioma
Basic Helix-Loop-Helix Transcription Factors
Aged, 80 and over
Brain Neoplasms
Astrocytoma
General Medicine
Middle Aged
Immunohistochemistry
Subtyping
ErbB Receptors
Hyaluronan Receptors
Female
Algorithms
Adult
medicine.medical_specialty
Histology
Oligodendroglioma
Nerve Tissue Proteins
World Health Organization
Pathology and Forensic Medicine
Young Adult
Proto-Oncogene Proteins
Glioma
Biomarkers, Tumor
medicine
Humans
protein expression
Aged
Retrospective Studies
Neoplasm Grading
c-Mer Tyrosine Kinase
business.industry
Receptor Protein-Tyrosine Kinases
Original Articles
Oligodendrocyte Transcription Factor 2
medicine.disease
nervous system diseases
Tissue Array Analysis
Tumor Suppressor Protein p53
Glioblastoma
business
Subjects
Details
- ISSN :
- 13652559 and 03090167
- Volume :
- 64
- Database :
- OpenAIRE
- Journal :
- Histopathology
- Accession number :
- edsair.doi.dedup.....fbc5f34bb004baf9c100e048da38634f