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HNRNPA2B1 Is a Mediator of m6A-Dependent Nuclear RNA Processing Events
- Source :
- Cell, vol 162, iss 6
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- N(6)-methyladenosine (m(6)A) is the most abundant internal modification of messenger RNA. While the presence of m(6)A on transcripts can impact nuclear RNA fates, a reader of this mark that mediates processing of nuclear transcripts has not been identified. We find that the RNA-binding protein HNRNPA2B1 binds m(6)A-bearing RNAs in vivo and in vitro and its biochemical footprint matches the m(6)A consensus motif. HNRNPA2B1 directly binds a set of nuclear transcripts and elicits similar alternative splicing effects as the m(6)A writer METTL3. Moreover, HNRNPA2B1 binds to m(6)A marks in a subset of primary miRNA transcripts, interacts with the microRNA Microprocessor complex protein DGCR8, and promotes primary miRNA processing. Also, HNRNPA2B1 loss and METTL3 depletion cause similar processing defects for these pri-miRNA precursors. We propose HNRNPA2B1 to be a nuclear reader of the m(6)A mark and to mediate, in part, this mark's effects on primary microRNA processing and alternative splicing. PAPERCLIP.
- Subjects :
- RNA Processing
Adenosine
Exonic splicing enhancer
Post-Transcriptional
RNA-binding protein
Biology
Methylation
Medical and Health Sciences
Article
General Biochemistry, Genetics and Molecular Biology
Cell Line
Microprocessor complex
Cell Line, Tumor
Heterogeneous-Nuclear Ribonucleoprotein Group A-B
Genetics
Humans
RNA Processing, Post-Transcriptional
Cell Nucleus
Tumor
Biochemistry, Genetics and Molecular Biology(all)
Alternative splicing
Intron
RNA-Binding Proteins
RNA
Biological Sciences
Molecular biology
Alternative Splicing
HEK293 Cells
RNA editing
Hela Cells
Transcriptome
Small nuclear RNA
HeLa Cells
Biotechnology
Developmental Biology
Subjects
Details
- ISSN :
- 00928674
- Volume :
- 162
- Database :
- OpenAIRE
- Journal :
- Cell
- Accession number :
- edsair.doi.dedup.....fbe380c58daf542293390ea96a3cff08