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Improved organotypic skin model with reduced quantity of monounsaturated ceramides by inhibiting stearoyl-CoA desaturase-1
- Source :
- 'Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids ', vol: 1866, pages: 158885-1-158885-7 (2021), Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 1866(4). ELSEVIER
- Publication Year :
- 2021
-
Abstract
- Full thickness models (FTM) are 3D in vitro skin cultures that resemble the native human skin (NHS) to a great extent. However, the barrier function of these skin models is reduced. The skin barrier is located in the stratum corneum (SC) and consists of corneocytes embedded in a lipid matrix. In this matrix, deviations in the composition of the FTMs lipid matrix may contribute to the impaired skin barrier when compared to NHS. One of the most abundant changes in lipid composition is an increase in monounsaturated lipids for which stearoyl-CoA desaturase-1 (SCD-1) is responsible. To improve the SC lipid composition, we reduced SCD-1 activity during the generation of the FTMs. These FTMs were subsequently assessed on all major aspects, including epidermal homeostasis, lipid composition, lipid organization, and barrier functionality. We demonstrate that SCD-1 inhibition was successful and resulted in FTMs that better mimic the lipid composition of FTMs to NHS by a significant reduction in monounsaturated lipids. In conclusion, this study demonstrates an effective approach to normalize SC monounsaturated lipid concentration and may be a valuable tool in further optimizing the FTMs in future studies.
- Subjects :
- Adult
Ceramide
Human skin
Matrix (biology)
Ceramides
Young Adult
030207 dermatology & venereal diseases
03 medical and health sciences
chemistry.chemical_compound
Organ Culture Techniques
0302 clinical medicine
Stratum corneum
medicine
Humans
Enzyme Inhibitors
Molecular Biology
Cells, Cultured
Barrier function
Skin
030304 developmental biology
0303 health sciences
Corneocyte
integumentary system
Chemistry
Cell Biology
In vitro
medicine.anatomical_structure
Biochemistry
Female
lipids (amino acids, peptides, and proteins)
Stearoyl-CoA desaturase-1
Stearoyl-CoA Desaturase
Subjects
Details
- Language :
- English
- ISSN :
- 13881981
- Volume :
- 1866
- Database :
- OpenAIRE
- Journal :
- Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
- Accession number :
- edsair.doi.dedup.....fbeafa8b4a87ac847c5db6917026cda5