Back to Search
Start Over
Pancreatic Islet Cells Express BST-1, a CD38-like Surface Molecule Having ADP-Ribosyl Cyclase Activity
- Source :
- Biochemical and Biophysical Research Communications. 219:941-946
- Publication Year :
- 1996
- Publisher :
- Elsevier BV, 1996.
-
Abstract
- Cyclic ADP-ribose (cADPR), a well-known stimulator of ca(2+) release from the intracellular Ca(2+) pool, has recently emerged as a potential regulator of insulin secretion in pancreatic beta cells. As recently described, BST-1 is a glycosyl-phosphatidylinositol (GPI)-anchored surface molecule that exhibits homology with CD38 and Aplysia ADP-ribosyl cyclase. Like CD38, BST-1 has both ADP-ribosyl cyclase and cADPR hydrolase activities. As a step toward elucidating the physiological role of cADPR in insulin secretion we examined whether BST-1 is expressed in pancreatic islet cells. Sensitive reverse transcription-polymerase chain reaction detected almost as abundant expression of BST-1 mRNA in pancreatic islets as CD38 mRNA. Immunohistochemical analyses utilizing mirror image sections revealed that BST-1 protein is expressed in a majority of the cells in pancreatic islets and that at least beta cells and, to an even greater extent, alpha cells express BST-1. These observations suggest the involvement of multiple enzymes in the regulation of cADPR concentrations in pancreatic islet cells.
- Subjects :
- Glycosylphosphatidylinositols
Biophysics
Gene Expression
CD38
Biology
GPI-Linked Proteins
Transfection
Polymerase Chain Reaction
Biochemistry
Cyclase
Islets of Langerhans
Mice
Antigens, CD
Aplysia
medicine
Animals
Humans
Point Mutation
RNA, Messenger
ADP-ribosyl Cyclase
N-Glycosyl Hydrolases
Molecular Biology
chemistry.chemical_classification
Mice, Inbred BALB C
Messenger RNA
Membrane Glycoproteins
Sequence Homology, Amino Acid
Pancreatic islets
Cell Biology
Flow Cytometry
ADP-ribosyl cyclase activity
ADP-ribosyl Cyclase 1
Antigens, Differentiation
Immunohistochemistry
Recombinant Proteins
Cell biology
medicine.anatomical_structure
Enzyme
chemistry
Antigens, Surface
Intracellular
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 219
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....fc02af89b7c717b6a2c3e2a07cdcd87b