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The effect of aspartate supplementation on the microbial composition and innate immunity on mice
- Source :
- Amino acids. 49(12)
- Publication Year :
- 2017
-
Abstract
- The study was conducted to investigate the changes of intestinal microbiota composition and innate immunity with different dietary dosages of aspartate (Asp) supplementation. Thirty-six female ICR mice were divided randomly to four groups and thereafter fed the basal diets (controls) or those supplemented with additional 0.5, 1.0 and 2.0% aspartate. After 2 week feeding, microbial composition in ileum and feces, gene expression of pro-inflammatory cytokine, and innate immune factors in ileum were determined. The ratio of Firmicutes: Bacteroidetes in ileum and feces decreased in 0.5 and 1.0% Asp-supplemented groups, whereas this ratio increased in feces in 2.0% Asp-supplemented group. Meanwhile, the gene expression of IL-17 and IFN-γ in ileum decreased in 1.0% Asp-supplemented group; the gene expression in ileum of Muc2 decreased in 0.5 and 1.0% Asp-supplemented groups. Dietary supplementation with 2.0% Asp enhanced the expression of pIgR and Crp1 as compared to the other three groups. The results indicated that dietary 1.0% Asp supplementation lowers the ratio of Firmicutes:Bacteroidetes, which affects the innate immunity by decreasing the gene expression of IL-17, IFN-γ, and Muc2 in ileum.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Firmicutes
Clinical Biochemistry
Ileum
Mucin 2
digestive system
Biochemistry
Polymerase Chain Reaction
Microbiology
03 medical and health sciences
Interferon-gamma
Mice
Internal medicine
RNA, Ribosomal, 16S
Gene expression
medicine
Animals
Feces
Regulation of gene expression
Aspartic Acid
Mice, Inbred ICR
Mucin-2
030109 nutrition & dietetics
Innate immune system
biology
Organic Chemistry
Interleukin-17
Bacteroidetes
biology.organism_classification
Immunity, Innate
Gastrointestinal Microbiome
030104 developmental biology
medicine.anatomical_structure
Endocrinology
Gene Expression Regulation
Dietary Supplements
Female
Subjects
Details
- ISSN :
- 14382199
- Volume :
- 49
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Amino acids
- Accession number :
- edsair.doi.dedup.....fc468ca7d5ef951f8a8ad6166937b1d0