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Author Correction: Identification of a binding protein for sesamin and characterization of its roles in plant growth

Authors :
Atsushi Okazawa
Ayako Furukawa
Yasuaki Kabe
Makoto Suematsu
Shoko Mori
Jun Murata
Toshiaki Azuma
Takehiro Watanabe
Honoo Satake
Katsuhito Hori
Eiichiro Ono
Masayuki Tera
Manabu Horikawa
Tomotsugu Koyama
Source :
Scientific Reports, Vol 10, Iss 1, Pp 1-2 (2020), Scientific Reports, Vol 10, Iss 1, Pp 1-1 (2020), Scientific Reports
Publication Year :
2020
Publisher :
Nature Publishing Group, 2020.

Abstract

Sesamin is a furofuran-type lignan that is found abundantly in seeds of Sesamum indicum (sesame) and has been widely accepted as a dietary supplement with positive effects on human health. The biological activity of sesamin in human cells and organs has been analysed extensively, although comparatively few studies show biological functions for sesamin in planta. Herein we screened sesamin-binding proteins (SBP) from sesame seedling extracts using sesamin-immobilized nano-beads. In subsequent peptide mass fingerprinting analyses, we identified a SBP, Steroleosin B, which is one of the membrane proteins found in oil bodies. In addition, pull-down assays and saturation transfer difference-nuclear magnetic resonance (STD-NMR) experiments demonstrated that sesamin binds directly to recombinant Steroleosin B in vitro. Finally, ectopic accumulations of sesamin and Steroleosin B in transgenic Arabidopsis thaliana plants induced severe growth defects including suppression of leaf expansion and root elongation. Collectively, these results indicate that sesamin influences tissue development in the presence of Steroleosin B.

Details

Language :
English
ISSN :
20452322
Volume :
10
Issue :
1
Database :
OpenAIRE
Journal :
Scientific Reports
Accession number :
edsair.doi.dedup.....fc4ba256389a2aeb19941a3f49e313ee
Full Text :
https://doi.org/10.1038/s41598-020-66086-7