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Respiratory inhibition of isolated mammalian mitochondria by salivary antifungal peptide histatin-5
- Source :
- Biochemical and biophysical research communications, 28 (2003): 1034–1040., info:cnr-pdr/source/autori:Petruzzelli R., Clementi M.E., Marini S., Coletta M., Di Stasio E., Giardina B., Misiti F./titolo:Respiratory inhibition of isolated mammalian mitochondria by salivary antifungal peptide histatin-5/doi:/rivista:Biochemical and biophysical research communications (Print)/anno:2003/pagina_da:1034/pagina_a:1040/intervallo_pagine:1034–1040/volume:28
- Publication Year :
- 2003
- Publisher :
- Academic Press Incorporated:6277 Sea Harbor Drive:Orlando, FL 32887:(800)543-9534, (407)345-4100, EMAIL: ap@acad.com, INTERNET: http://www.idealibrary.com, Fax: (407)352-3445, 2003.
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Abstract
- Histatin-5 is a peptide secreted in the human saliva, which possesses powerful antifungal activity. Previous studies have shown that this peptide exerts its candidacidal activity, through the inhibition of both mitochondrial respiration and the formation of reactive oxygen species. The purpose of the present study was to investigate the biological consequences of histatin-5 action on mammalian mitochondria to verify if the toxic mechanism exerted on mitochondria from Candida albicans is an exclusive for fungal cells. Moreover, hypothesising that the damage exerted on mitochondria may induce programmed cellular death pathways, we evaluated two main markers of apoptosis: the mitochondrial membrane potential (DeltaPsi) and the release of cytochrome c. The results obtained show that exposure of isolated mammalian mitochondria to histatin-5 determines: (i) a large inhibition of the respiratory chain at the level of complex I, (ii) a slight decrease in the mitochondrial membrane potential, and (iii) no release of cytochrome c.
- Subjects :
- Antifungal Agents
Histatin-5
Cytochrome
Cellular respiration
Cell Respiration
Biophysics
Respiratory chain
Apoptosis
Histatins
Mitochondrion
Biochemistry
Membrane Potentials
Rats, Sprague-Dawley
Salivary peptides
Animals
Salivary Proteins and Peptides
Settore BIO/10
Mitochondria
Molecular Biology
Cells, Cultured
chemistry.chemical_classification
Reactive oxygen species
biology
Dose-Response Relationship, Drug
Cytochromes c
Cell Biology
Cell biology
Rats
chemistry
Histatin
biology.protein
Apoptosome
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications, 28 (2003): 1034–1040., info:cnr-pdr/source/autori:Petruzzelli R., Clementi M.E., Marini S., Coletta M., Di Stasio E., Giardina B., Misiti F./titolo:Respiratory inhibition of isolated mammalian mitochondria by salivary antifungal peptide histatin-5/doi:/rivista:Biochemical and biophysical research communications (Print)/anno:2003/pagina_da:1034/pagina_a:1040/intervallo_pagine:1034–1040/volume:28
- Accession number :
- edsair.doi.dedup.....fc5904bb4324fdc4f7bb2ea30e8250c2