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Long-Term Functional Stability of Functional Nucleic Acid–Gold Nanoparticle Conjugates with Different Secondary Structures
- Source :
- Langmuir. 35:11791-11798
- Publication Year :
- 2019
- Publisher :
- American Chemical Society (ACS), 2019.
-
Abstract
- Thiolated functional nucleic acid-gold nanoparticle conjugates (FNA-AuNPs) are the core recognition elements in biosensors. The long-term functional stability (LTFS) is critical for their practical applications and, however, has been overlooked. Here we report on the huge effects of multiple experimental factors on LTFS, including spacer- and buffer-composition, secondary structures of FNAs, and surface blocking. We quantitatively determined these effects by measuring the relative hybridization capacity (RHC, the relative amount of complementary DNA hybridized with the same amount of conjugates) for linear DNA-AuNP or the relative signal change generated by their function (RSC-F) for molecular beacon (MB) and G-quadruplex (G4)-AuNPs. There is a positive relationship between the spacer affinity [oligoadenine (A10) > oligothymine (T10) > oligoethlyene glycol (EG18)] of the linear DNA probes and the LTFS. The LTFS of linear DNA-AuNP in phosphate buffer (PB) was much better than that in Good’s buffers such as...
- Subjects :
- Surface Properties
Metal Nanoparticles
Nanoparticle
02 engineering and technology
010402 general chemistry
01 natural sciences
Molecular beacon
Complementary DNA
Functional stability
Electrochemistry
General Materials Science
Particle Size
Spectroscopy
Chemistry
Hybridization probe
DNA
Surfaces and Interfaces
021001 nanoscience & nanotechnology
Condensed Matter Physics
Combinatorial chemistry
0104 chemical sciences
G-Quadruplexes
Nucleic acid
Gold
0210 nano-technology
Biosensor
Conjugate
Subjects
Details
- ISSN :
- 15205827 and 07437463
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Langmuir
- Accession number :
- edsair.doi.dedup.....fc6e67592e10f7a208c16a6aaedd6472