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Vascular Endothelial Growth Factor Induction of Muscle-Derived Stem Cells Enhances Vascular Phenotype While Preserving Myogenic Potential
- Source :
- Annals of plastic surgery. 79(4)
- Publication Year :
- 2017
-
Abstract
- BACKGROUND Previous work by our group and other laboratories have revealed that muscle-derived stem cells (MDSCs) may contain both myogenic and endothelial progenitors, making MDSCs a promising option for skeletal muscle regeneration. The purpose of this study was to investigate the impact of vascular endothelial growth factor (VEGF) induction on the vascular and myogenic potential of MDSCs. METHODS Muscle-derived stem cells were isolated from 4- to 8-week-old C57BL/6J mice using a preplate technique and recombinant human VEGFa was used as the induction agent. Cellular proliferation and migration were assessed using serial imaging and wound healing assays, respectively. Myosin heavy chain staining was performed to assess MDSC myotube formation. Vascular potential of MDSCs was measured by expression of CD31 and in vitro capillary tube formation. RESULTS Vascular endothelial growth factor stimulation led to a dose-dependent increase in MDSC proliferation (P < 0.05) and migration kinetics (P < 0.01). Control MDSCs had low levels of baseline expression of CD31, which was significantly upregulated by VEGF stimulation. Similarly, MDSCs demonstrated a basal capability for capillary tube formation, which was significantly increased after VEGF induction as evidenced by increased branches (5.91 ± 0.58 vs 9.23 ± 0.67, P < 0.01) and total tube length (11.73 ± 0.97 vs 18.62 ± 1.57 mm, P < 0.01). Additionally, the myogenic potential of MDSCs as measured by fusion index remained unchanged with increasing concentration of VEGF up to 250 ng/mL (P = 0.77). CONCLUSIONS Vascular endothelial growth factor induction enhances MDSC proliferation, migration, and endothelial phenotypes without negatively impacting myogenic potential. These results suggest that VEGF stimulation may improve vascularization of MDSC-based strategies for skeletal muscle regeneration.
- Subjects :
- 0301 basic medicine
CD31
Male
Vascular Endothelial Growth Factor A
Neovascularization, Physiologic
Muscle Development
Andrology
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Cell Movement
Medicine
Animals
Regeneration
Muscle, Skeletal
Cell Proliferation
Tissue Engineering
business.industry
Stem Cells
Skeletal muscle
Recombinant Proteins
Vascular endothelial growth factor B
Vascular endothelial growth factor
Mice, Inbred C57BL
Vascular endothelial growth factor A
030104 developmental biology
medicine.anatomical_structure
Phenotype
Vascular endothelial growth factor C
chemistry
030220 oncology & carcinogenesis
Surgery
Stem cell
business
Wound healing
Subjects
Details
- ISSN :
- 15363708
- Volume :
- 79
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Annals of plastic surgery
- Accession number :
- edsair.doi.dedup.....fc8f5ee68bf40b51c00e011d215c7336