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Failure to Demonstrate Increased Protein Turnover and Intracellular Proteinase Activity in Livers of Mice with Streptozotocin-induced Diabetes
- Source :
- Diabetes. 29:648-654
- Publication Year :
- 1980
- Publisher :
- American Diabetes Association, 1980.
-
Abstract
- SUMMARY The effect of streptozotocin-induced diabetes on the turnover of acidic and basic mouse liver cytosol proteins was assessed. Previous work by others had demonstrated that acidic proteins are degraded more rapidly than basic proteins in livers of normal but not in severely diabetic animals. In this study, a milder form of diabetes was investigated to determine whether insulin-dependent diabetes in the absence of starvation, large weight losses, and impending death results in fundamental changes in degradation of the normally labile (acidic) proteins and stable (basic) proteins. The relative rates of degradation of liver proteins were measured by a double-isotope technique and by the loss of protein radiolabeled with [14C]-bi-carbonate. The relative rates of synthesis of proteins were estimated by incorporations of [3H]-leucine. No fundamental change in the relative rates of synthesis or degradation of acidic and basic proteins was observed. There was a general decrease in the incorporation of radiolabeled amino acids in the diabetic state and an increase in the specific activity of some amino acid–metabolizing enzymes, indicating changes in amino acid metabolism in liver. A study of the quantity and subcellular distribution of several liver cell proteinases revealed little if any changes in the proteolytic machinery of liver cells in this form of insulin-dependent diabetes. Thus, the fundamental changes in protein degradation seen in severe diabetes are not observed in a less severe form of the disease.
- Subjects :
- Male
medicine.medical_specialty
Endocrinology, Diabetes and Metabolism
Biology
Protein degradation
Diabetes Mellitus, Experimental
Mice
Cytosol
Leucine
Internal medicine
Internal Medicine
medicine
Animals
chemistry.chemical_classification
Mice, Inbred BALB C
Liver cell
Protein turnover
Proteins
Streptozotocin
Amino acid
Bicarbonates
Kinetics
Enzyme
Endocrinology
Liver
chemistry
Biochemistry
Organ Specificity
Specific activity
Intracellular
Peptide Hydrolases
Subcellular Fractions
medicine.drug
Subjects
Details
- ISSN :
- 1939327X and 00121797
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- Diabetes
- Accession number :
- edsair.doi.dedup.....fcb85d5614fe5f2ba35d57d665f719dd