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Increasing versatility of the DNA vaccines through modification of the subcellular location of plasmid-encoded antigen expression in the in vivo transfected cells
- Source :
- PLoS ONE, Vol 8, Iss 10, p e77426 (2013), BASE-Bielefeld Academic Search Engine, Repositorio de Resultados de Investigación del INIA, INIA: Repositorio de Resultados de Investigación del INIA, PLoS ONE, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA
- Publication Year :
- 2013
- Publisher :
- Public Library of Science (PLoS), 2013.
-
Abstract
- The route of administration of DNA vaccines can play a key role in the magnitude and quality of the immune response triggered after their administration. DNA vaccines containing the gene of the membrane-anchored glycoprotein (gpG) of the fish rhabdoviruses infectious haematopoietic necrosis virus (IHNV) or viral haematopoietic septicaemia virus (VHSV), perhaps the most effective DNA vaccines generated so far, confer maximum protection when injected intramuscularly in contrast to their low efficacy when injected intraperitoneally. In this work, taking as a model the DNA vaccine against VHSV, we focused on developing a more versatile DNA vaccine capable of inducing protective immunity regardless of the administration route used. For that, we designed two alternative constructs to gpG1-507 (the wild type membrane-anchored gpG of VHSV) encoding either a soluble (gpG1-462) or a secreted soluble (gpGLmPle20-462) form of the VHSV-gpG. In vivo immunisation/challenge assays showed that only gpGLmPle20-462 (the secreted soluble form) conferred protective immunity against VHSV lethal challenge via both intramuscular and intraperitoneal injection, being this the first description of a fish viral DNA vaccine that confers protection when administered intraperitoneally. Moreover, this new DNA vaccine construct also conferred protection when administered in the presence of an oil adjuvant suggesting that DNA vaccines against rhabdoviruses could be included in the formulation of current multicomponent-intaperitoneally injectable fish vaccines formulated with an oil adjuvant. On the other hand, a strong recruitment of membrane immunoglobulin expressing B cells, mainly membrane IgT, as well as t-bet expressing T cells, at early times post-immunisation, was specifically observed in the fish immunised with the secreted soluble form of the VHSV-gpG protein; this may indicate that the subcellular location of plasmid-encoded antigen expression in the in vivo transfected cells could be an important factor in determining the ways in which DNA vaccines prime the immune response. © 2013 Martinez-Lopez et al.
- Subjects :
- Oncorhynchus
Gene Expression
lcsh:Medicine
Antibodies, Viral
DNA vaccination
Virus
Cell Line
Fish Diseases
Plasmid
Immune system
Antigen
Hemorrhagic Septicemia, Viral
Vaccines, DNA
Animals
antibodies
lcsh:Science
Antigens, Viral
Viral Structural Proteins
trout
B cells
Multidisciplinary
biology
Viral Vaccine
lcsh:R
Viral Vaccines
vaccines
Virology
cell cultures
Cell culture
biology.protein
Immunization
lcsh:Q
enzyme-linked immunity
Antibody
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....fcb8b34b3a0fe01ac6fe4ef47c158a58