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Increasing versatility of the DNA vaccines through modification of the subcellular location of plasmid-encoded antigen expression in the in vivo transfected cells

Authors :
Regla María Medina-Gali
Maria del Mar Ortega-Villaizan
Luis Perez
Pablo Garcia-Valtanen
Amparo Estepa
Julio Coll
A. Martinez-Lopez
Veronica Chico
Martinez-Lopez, Alicia
Garcia-Valtanen, Pablo
Del, Mar Ortega-Villaizan Maria
Chico, Verónica
Medina-Gali, Regla Maria
Perez, Luis
Coll, Julio
Estepa, Amparo
Source :
PLoS ONE, Vol 8, Iss 10, p e77426 (2013), BASE-Bielefeld Academic Search Engine, Repositorio de Resultados de Investigación del INIA, INIA: Repositorio de Resultados de Investigación del INIA, PLoS ONE, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA
Publication Year :
2013
Publisher :
Public Library of Science (PLoS), 2013.

Abstract

The route of administration of DNA vaccines can play a key role in the magnitude and quality of the immune response triggered after their administration. DNA vaccines containing the gene of the membrane-anchored glycoprotein (gpG) of the fish rhabdoviruses infectious haematopoietic necrosis virus (IHNV) or viral haematopoietic septicaemia virus (VHSV), perhaps the most effective DNA vaccines generated so far, confer maximum protection when injected intramuscularly in contrast to their low efficacy when injected intraperitoneally. In this work, taking as a model the DNA vaccine against VHSV, we focused on developing a more versatile DNA vaccine capable of inducing protective immunity regardless of the administration route used. For that, we designed two alternative constructs to gpG1-507 (the wild type membrane-anchored gpG of VHSV) encoding either a soluble (gpG1-462) or a secreted soluble (gpGLmPle20-462) form of the VHSV-gpG. In vivo immunisation/challenge assays showed that only gpGLmPle20-462 (the secreted soluble form) conferred protective immunity against VHSV lethal challenge via both intramuscular and intraperitoneal injection, being this the first description of a fish viral DNA vaccine that confers protection when administered intraperitoneally. Moreover, this new DNA vaccine construct also conferred protection when administered in the presence of an oil adjuvant suggesting that DNA vaccines against rhabdoviruses could be included in the formulation of current multicomponent-intaperitoneally injectable fish vaccines formulated with an oil adjuvant. On the other hand, a strong recruitment of membrane immunoglobulin expressing B cells, mainly membrane IgT, as well as t-bet expressing T cells, at early times post-immunisation, was specifically observed in the fish immunised with the secreted soluble form of the VHSV-gpG protein; this may indicate that the subcellular location of plasmid-encoded antigen expression in the in vivo transfected cells could be an important factor in determining the ways in which DNA vaccines prime the immune response. © 2013 Martinez-Lopez et al.

Details

Language :
English
ISSN :
19326203
Volume :
8
Issue :
10
Database :
OpenAIRE
Journal :
PLoS ONE
Accession number :
edsair.doi.dedup.....fcb8b34b3a0fe01ac6fe4ef47c158a58