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High resolution UPLC-MS/MS profiling of polyphenolics in the methanol extract of Syzygium samarangense leaves and its hepatoprotective activity in rats with CCl4-induced hepatic damage
- Source :
- Food and Chemical Toxicology. 113:145-153
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Oxidative stress plays a crucial role in the development of several liver diseases. Many natural polyphenols can attenuate oxidative stress and liver injury. In this study, a phytochemical profiling of a methanol extract from leaves of Syzygium samarangense revealed 92 compounds belonging to flavonoids, phenolic acids, condensed tannins, and ellagitannins. The S. samarangense extract exhibited a noticeable antioxidant activity with an EC50 of 5.80 μg/mL measured by DPPH scavenging capacity assay, 2632 Trolox equivalents, 10 mM Fe2+ equivalents/mg of samples by TEAC and FRAP assays, respectively. The total phenolic content was 419 mg gallic acid equivalent GAE/g extract. In a cell-based model (HaCaT cells), the extract completely inhibited ROS production induced by UVA, and prevented GSH-depletion and p38 phosphorylation. In addition, the extract exhibited a substantial antioxidant and hepatoprotective activities in CCl4-treated rats, with an increase in GSH (reduced glutathione) and SOD (superoxide dismutase) activities by 84.75 and 26.27%, respectively, and a decrease of 19.08, 63.05, 52.21, 37.00, 13.26, and 15.15% in MDA, ALT, AST, TB (total bilirubin), TC (total cholesterol), and TG (total glycerides), respectively. These results were confirmed by histopathological analyses. We believe that Syzygium samarangense is a good candidate for further evaluation as an antioxidant and liver protecting drug.
- Subjects :
- 0301 basic medicine
Antioxidant
medicine.medical_treatment
HaCaT cell
Hepatoprotective activity
Toxicology
medicine.disease_cause
01 natural sciences
Superoxide dismutase
03 medical and health sciences
chemistry.chemical_compound
medicine
Gallic acid
Food science
biology
010405 organic chemistry
General Medicine
Glutathione
Carbon tetrachloride toxicity
HR-UPLC-MS/MS
0104 chemical sciences
030104 developmental biology
chemistry
Phytochemical
Polyphenol
biology.protein
Trolox
Syzygium samarangense
Oxidative stress
Food Science
Subjects
Details
- ISSN :
- 02786915
- Volume :
- 113
- Database :
- OpenAIRE
- Journal :
- Food and Chemical Toxicology
- Accession number :
- edsair.doi.dedup.....fcc7d006e0693758536a12bd9481f2bc