Back to Search
Start Over
OmGOGAT-disruption in the ericoid mycorrhizal fungus Oidiodendron maius induces reorganization of the N pathway and reduces tolerance to heavy-metals
- Source :
- Fungal Genetics and Biology, Fungal Genetics and Biology, Elsevier, 2014, 71, pp.1-8, Fungal Genetics and Biology, Elsevier, 2014, 71, pp.1-8. ⟨10.1016/j.fgb.2014.08.003⟩, Fungal genetics and biology, 71 (2014): 1–8. doi:10.1016/j.fgb.2014.08.003, info:cnr-pdr/source/autori:Khouja H. R., Daghino S., Abba S., Boutaraa F., Chalot M., Blaudez D., Martino E., Perotto S./titolo:OmGOGAT-disruption in the ericoid mycorrhizal fungus Oidiodendron maius induces reorganization of the N pathway and reduces tolerance to heavy-metals/doi:10.1016%2Fj.fgb.2014.08.003/rivista:Fungal genetics and biology (Print)/anno:2014/pagina_da:1/pagina_a:8/intervallo_pagine:1–8/volume:71
- Publication Year :
- 2014
- Publisher :
- HAL CCSD, 2014.
-
Abstract
- Mycorrhizal fungi are key mediators of soil-to-plant movement of mineral nutrients, including essential and non-essential metals. In soil conditions that facilitate mobilization of metal ions, potentially toxic metals can interfere with nitrogen metabolism in both plants and microorganisms. Less is known about possible relationships between nitrogen metabolism and responses to heavy metals. Aim of this study was to investigate this aspect in the ericoid mycorrhizal fungus Oidiodendron maius strain Zn, a metal tolerant ascomycete. Growth of O. maius Zn on zinc and cadmium containing media was significantly affected by the nitrogen source. Screening of a library of O. maius Zn random genetic transformants for sensitivity to heavy metals (zinc and cadmium) and oxidative stress (menadione) yielded a mutant strain that carried a partial deletion of the glutamate synthase (NADH-GOGAT EC 1.4.1.14) gene and its adjacent gene, the APC15 subunit of the anaphase promoting complex. Comparison of WT and OmGOGAT-OmAPC15 mutant strains indicated an impaired N-metabolism and altered stress tolerance, and assays on the OrnAPC15-recomplemented strains ascribed the observed phenotypes to the deletion in the OmGOGAT gene. OmGOGAT disruption modified the nitrogen pathway, with a strong reduction of the associated glutamine synthetase (GS, EC 6.3.1.2) activity and an up-regulation of the alternative NADP-glutamate dehydrogenase (NADP-GDH, EC 1.4.1.4) pathway for glutamate biosynthesis. Unless they were supplemented with glutamine, O. maius Zn transformants lacking OmCOGAT were very sensitive to zinc. These results highlight the importance of nitrogen metabolism not only for nitrogen assimilation and transformation, but also for stress tolerance. For mycorrhizal fungi, such as O. maius, this may bear consequences not only to the fungus, but also to the host plant. (C) 2014 Elsevier Inc. All rights reserved.
- Subjects :
- 0106 biological sciences
Nitrogen
Nitrogen assimilation
Mutant
Vaccinium myrtillus
chemistry.chemical_element
Zinc
01 natural sciences
Microbiology
Anaphase-Promoting Complex-Cyclosome
Glutamine synthetase
[ SDV.EE ] Life Sciences [q-bio]/Ecology, environment
03 medical and health sciences
Transformation, Genetic
Ascomycota
Glutamate-Ammonia Ligase
Mycorrhizae
Glutamate synthase
Genetics
Nitrogen metabolism
Insertional mutagenesis
Mycorrhizal fungi
030304 developmental biology
2. Zero hunger
[SDV.EE]Life Sciences [q-bio]/Ecology, environment
0303 health sciences
Cadmium
biology
Glutamate Synthase
Heavy metal tolerance
15. Life on land
biology.organism_classification
Glutamine
Oxidative Stress
Protein Subunits
chemistry
Biochemistry
biology.protein
Glutamate Dehydrogenase (NADP+)
Gene Deletion
Metabolic Networks and Pathways
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 10871845 and 10960937
- Database :
- OpenAIRE
- Journal :
- Fungal Genetics and Biology, Fungal Genetics and Biology, Elsevier, 2014, 71, pp.1-8, Fungal Genetics and Biology, Elsevier, 2014, 71, pp.1-8. ⟨10.1016/j.fgb.2014.08.003⟩, Fungal genetics and biology, 71 (2014): 1–8. doi:10.1016/j.fgb.2014.08.003, info:cnr-pdr/source/autori:Khouja H. R., Daghino S., Abba S., Boutaraa F., Chalot M., Blaudez D., Martino E., Perotto S./titolo:OmGOGAT-disruption in the ericoid mycorrhizal fungus Oidiodendron maius induces reorganization of the N pathway and reduces tolerance to heavy-metals/doi:10.1016%2Fj.fgb.2014.08.003/rivista:Fungal genetics and biology (Print)/anno:2014/pagina_da:1/pagina_a:8/intervallo_pagine:1–8/volume:71
- Accession number :
- edsair.doi.dedup.....fcdd419644b904eb9cce621758126bde
- Full Text :
- https://doi.org/10.1016/j.fgb.2014.08.003⟩