Nucleotide resolution profiling of m7G tRNA modification by TRAC-Seq

Precise identification of sites of RNA modification is key to studying the functional role of such modifications in the regulation of gene expression and for elucidating relevance to diverse physiological processes. tRNA reduction and cleavage sequencing (TRAC-Seq) is a chemically based approach for the unbiased global mapping of 7-methylguansine (m(7)G) modification of tRNAs at single-nucleotide resolution throughout the tRNA transcriptome. m(7)G TRAC-Seq involves the treatment of size-selected (