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A comprehensive chemoselective and enantioselective 2D-HPLC set-up for fast enantiomer analysis of a multicomponent mixture of derivatized amino acids

Authors :
M. Chlup
Wolfgang Lindner
F. Meier
Michael Lämmerhofer
B. Müller
Thomas Welsch
A. Köhne
Christoph Schmidtkunz
Source :
Analytical and Bioanalytical Chemistry. 388:1717-1724
Publication Year :
2007
Publisher :
Springer Science and Business Media LLC, 2007.

Abstract

A feasibility study on the fast enantioselective two-dimensional HPLC separation of racemic amino acid derivatives is presented. The method involves the on-line coupling of a narrow-bore C18 RP column in the first dimension to a short enantioselective column based on nonporous 1.5 microm particles modified with quinidine carbamate as chiral selector in the second dimension. Conceptually, the system was designed to enable both time-controlled repeated transfer of fractions of the eluate and detector-controlled transfer of selected fractions from column 1 to column 2. To avoid volume overloading of the second chiral column, a narrow-bore reversed phase column was installed in the first dimension. Due to the fast (less than 1.5 minutes) enantiomer separation that occurs in the second dimension, the overall analysis time for the two-dimensional separation of a mixture of nine racemic 3,5-dinitrobenzoyl amino acids was optimized at 16 minutes.

Details

ISSN :
16182650 and 16182642
Volume :
388
Database :
OpenAIRE
Journal :
Analytical and Bioanalytical Chemistry
Accession number :
edsair.doi.dedup.....fd05d7d8b300e8ac18b85b8191198524
Full Text :
https://doi.org/10.1007/s00216-007-1399-4