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High Concentration of Insulin Promotes Apoptosis of Primary Cultured Rat Ovarian Granulosa Cells Via Its Increase in Extracellular HMGB1
- Source :
- Reproductive Sciences. 22:271-277
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- Polycystic ovary syndrome (PCOS) is a common endocrine disorder affecting women of reproductive age. Insulin resistance/hyperinsulinemia is a prevalent finding in women with PCOS, which indicates that insulin resistance/hyperinsulinemia may be an important player in the pathogenesis of the PCOS. However, the underlying mechanism of insulin resistance/hyperinsulinemia on the pathogenesis of the PCOS remains elusive. In this study, we found an increased high-mobility group box 1 (HMGB1) in the serum from women with PCOS having insulin resistance/hyperinsulinemia. Furthermore, we discovered that high concentration of insulin, which mimics insulin resistance model, promoted apoptosis in primary cultured rat ovarian granulosa cells (GCs) via its effect on the increase in extracellular HMGB1. Our data presented the first evidence that increased HMGB1 induced by insulin resistance/hyperinsulinemia promoted apoptosis of ovarian GCs, which provided new molecular basis for the PCOS pathogenesis.
- Subjects :
- endocrine system
medicine.medical_specialty
Time Factors
endocrine system diseases
medicine.medical_treatment
Apoptosis
Transfection
HMGB1
Rats, Sprague-Dawley
Pathogenesis
Insulin resistance
Internal medicine
medicine
Hyperinsulinemia
Animals
Humans
Insulin
Endocrine system
HMGB1 Protein
Cells, Cultured
Granulosa Cells
Dose-Response Relationship, Drug
biology
Polycystic ovary syndrome (PCOS)
nutritional and metabolic diseases
Obstetrics and Gynecology
medicine.disease
Polycystic ovary
female genital diseases and pregnancy complications
Up-Regulation
Endocrinology
Case-Control Studies
biology.protein
Female
RNA Interference
Insulin Resistance
hormones, hormone substitutes, and hormone antagonists
Polycystic Ovary Syndrome
Signal Transduction
Subjects
Details
- ISSN :
- 19337205 and 19337191
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Reproductive Sciences
- Accession number :
- edsair.doi.dedup.....fd0f0c147a7aa911a9031b69e1dfb724