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The Anti-colitis Effect of Schisandra chinensis Polysaccharide Is Associated With the Regulation of the Composition and Metabolism of Gut Microbiota
- Source :
- Frontiers in Cellular and Infection Microbiology, Frontiers in Cellular and Infection Microbiology, Vol 10 (2020)
- Publication Year :
- 2020
- Publisher :
- Frontiers Media S.A., 2020.
-
Abstract
- Background: The pathogenesis of inflammatory bowel disease (IBD) is linked to an intricate association of environmental, microbial, and host-related factors. Polysaccharide affects host immunity by regulating the composition and metabolism of gut microbiota is the common mechanism of disease resistance. However, the efficacy and mechanism of Schisandra chinensis polysaccharide (SCP) in the treatment of inflammatory bowel disease have not been studied. Objective: To explore the effect and mechanism of SCP on dextran sodium sulfate (DSS) - induced ulcerative colitis (UC) in mice. Materials/Methods: In this study, we established a mouse model of UC, and used SCP for treatment intervention. The biochemical indexes related to inflammation were determined by ELISA kit, and the therapeutic effect of SCP on UC was clarified. Then, 16S rDNA sequencing was used to study the effect of SCP on the composition and diversity of gut microbiota. At the same time, GC-MS was used to determine the content of short chain fatty acids in intestinal contents. Finally, the relationship among gut microbiota, short chain fatty acids and inflammatory factors was analyzed, and to comprehensively explain the effect and mechanism of SCP on UC. Results: The results showed that SCP could significantly improve the physiological state of UC mice and regulate the level of inflammatory factors to normal levels. Meanwhile, SCP could significantly regulate the imbalance of gut microbiota and increase the content of SCFAs. In addition, the results of the correlation between gut microbiota and SCFAs showed that butyric acid, isobutyric acid and valeric acid had the highest correlation with gut microbiota. Conclusion: In conclusion, this research showed that SCP can inhibit inflammatory bowel disease by regulating the composition and metabolism of gut microbiota, and indicating that SCP may be used as adjuvant therapy for IBD patients.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Schisandra chinensis
030106 microbiology
Immunology
lcsh:QR1-502
short chain fatty acid
Inflammation
Gut flora
Microbiology
Inflammatory bowel disease
digestive system
lcsh:Microbiology
Pathogenesis
Butyric acid
03 medical and health sciences
chemistry.chemical_compound
Mice
Cellular and Infection Microbiology
Polysaccharides
medicine
Animals
Humans
Colitis
Original Research
ulcerative colitis
Schisandra
biology
gut microbiota
Short-chain fatty acid
Dextran Sulfate
biology.organism_classification
medicine.disease
Ulcerative colitis
Gastrointestinal Microbiome
Disease Models, Animal
030104 developmental biology
Infectious Diseases
chemistry
inflammation
polysaccharide
Colitis, Ulcerative
medicine.symptom
Subjects
Details
- Language :
- English
- ISSN :
- 22352988
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Frontiers in Cellular and Infection Microbiology
- Accession number :
- edsair.doi.dedup.....fd0fa7ec706e31a0b9785d478bb74bac