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Modulation of immunological responses by aqueous extract of Datura stramonium L. seeds on cyclophosphamide-induced immunosuppression in Wistar rats
- Source :
- BMC Immunology. 23
- Publication Year :
- 2022
- Publisher :
- Springer Science and Business Media LLC, 2022.
-
Abstract
- Background Datura stramonium L. (Solanaceae) is used traditionally in west Africa to treat asthma, epilepsy, rheumatoid arthritis, filariasis microbial infections and conjunctivitis. This study investigated the immunomodulatory effects of aqueous seed extract of D. stramonium L. (ASEDS) on Wistar rats. Methods Thirty Wistar albino rats (180–200 g) were randomized into 6 groups (n = 5). Group 1 received distilled water only. Rats in groups 2–6 were pretreated with 10 mg/kg body weight (b.w.) Cyclophosphamide orally for 27-days to induce immunosuppression. Thereafter, they received treatment orally for 28 days as follows: Group 2 (distilled water), group 3 (5 mg/kg b.w. Levamisole), groups 4–6 (60, 90 and 120 mg/kg b.w. ASEDS, respectively). HPLC was used to determine major compounds in ASEDS. The effects of ASEDS on immune cells, immunoglobulins A, G and M levels, lipoproteins, and antioxidant status of rats were evaluated. Results ASEDS indicated high content of Acutumine, Quinine, Catechin, Chlorogenic acid, Gallic acid, Quercetin, Vanillic acid, Luteolin, Formosanin C, Saponin, Cyanidin, Tannic acid, 3-Carene, Limonene and α-terpineol. Cyclophosphamide triggered significant (p p Conclusions The results obtained from the study showed the immunomodulatory activity of ASEDS, thereby indicating its potential in immunostimulatory drug discovery.
- Subjects :
- Datura stramonium
Cyclophosphamide
medicine.medical_treatment
Immunology
Pharmacology
Antioxidants
Catechin
Gallic Acid
medicine
Animals
Rats, Wistar
Luteolin
Immunosuppression Therapy
Vanillic Acid
Aqueous extract
Glutathione Peroxidase
Quinine
biology
Plant Extracts
Superoxide Dismutase
Chemistry
Water
Immunosuppression
Vitamins
Saponins
Catalase
biology.organism_classification
Rats
Immunoglobulin A
Levamisole
Immunoglobulin G
Seeds
Quercetin
Chlorogenic Acid
Lipoproteins, HDL
Tannins
Limonene
medicine.drug
Subjects
Details
- ISSN :
- 14712172
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- BMC Immunology
- Accession number :
- edsair.doi.dedup.....fd42d5bff3654238a44d942a346eb4c3