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Differential Spatio-Temporal Regulation of T-Box Gene Expression by microRNAs during Cardiac Development

Authors :
Diego Franco
Mohamad Alzein
Francisco Hernández-Torres
Amelia Aránega
Carlos García-Padilla
Jorge N. Domínguez
Estefanía Lozano-Velasco
Source :
Journal of Cardiovascular Development and Disease, Volume 8, Issue 5, Pages: 56, Journal of Cardiovascular Development and Disease, Vol 8, Iss 56, p 56 (2021)
Publication Year :
2021
Publisher :
MDPI, 2021.

Abstract

Cardiovascular development is a complex process that starts with the formation of symmetrically located precardiac mesodermal precursors soon after gastrulation and is completed with the formation of a four-chambered heart with distinct inlet and outlet connections. Multiple transcriptional inputs are required to provide adequate regional identity to the forming atrial and ventricular chambers as well as their flanking regions; i.e., inflow tract, atrioventricular canal, and outflow tract. In this context, regional chamber identity is widely governed by regional activation of distinct T-box family members. Over the last decade, novel layers of gene regulatory mechanisms have been discovered with the identification of non-coding RNAs. microRNAs represent the most well-studied subcategory among short non-coding RNAs. In this study, we sought to investigate the functional role of distinct microRNAs that are predicted to target T-box family members. Our data demonstrated a highly dynamic expression of distinct microRNAs and T-box family members during cardiogenesis, revealing a relatively large subset of complementary and similar microRNA–mRNA expression profiles. Over-expression analyses demonstrated that a given microRNA can distinctly regulate the same T-box family member in distinct cardiac regions and within distinct temporal frameworks, supporting the notion of indirect regulatory mechanisms, and dual luciferase assays on Tbx2, Tbx3 and Tbx5 3′ UTR further supported this notion. Overall, our data demonstrated a highly dynamic microRNA and T-box family members expression during cardiogenesis and supported the notion that such microRNAs indirectly regulate the T-box family members in a tissue- and time-dependent manner.

Details

Language :
English
ISSN :
23083425
Volume :
8
Issue :
5
Database :
OpenAIRE
Journal :
Journal of Cardiovascular Development and Disease
Accession number :
edsair.doi.dedup.....fd4c50027185d3ba0b03bfc769c7b3eb